| Immunogen | KLH-conjugatedsyntheticlinearpeptidecorrespondingtothehypervariableregionofRas,H,palmitoylated. |
| Epitope | hypervariableregion |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Rabbit |
| Specificity | ThisantibodydetectsthehypervariableregionRas,HPalmitoylated. |
| SpeciesReactivity | |
| SpeciesReactivityNote | Human.ExpectedtoreactwithRat,MouseandChickenbasedonsequencehomology. |
| AntibodyType | PolyclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | ThisgenebelongstotheRasoncogenefamily,whosemembersarerelatedtothetransforminggenesofmammaliansarcomaretroviruses.Theproductsencodedbythesegenesfunctioninsignaltransductionpathways.TheseproteinscanbindGTPandGDP,andtheyhaveintrinsicGTPaseactivity.Thisproteinundergoesacontinuouscycleofde-andre-palmitoylation,whichregulatesitsrapidexchangebetweentheplasmamembraneandtheGolgiapparatus.MutationsinthisgenecauseCostellosyndrome,adisease characterizedbyincreasedgrowthattheprenatalstage,growthdeficiencyatthepostnatalstage,predispositiontotumorformation,mentalretardation,skinandmusculoskeletalabnormalities,distinctivefacialappearanceandcardiovascularabnormalities.Defectsinthisgeneareimplicatedinavarietyofcancers,includingbladdercancer,follicularthyroidcancer,andoralsquamouscellcarcinoma.Multipletranscriptvariants,whichencodedifferentisoforms,havebeenidentifiedforthisgene.[providedbyRefSeq].
|
| GeneSymbol | - ALPS4
- HRAS1
- N-ras
- NRAS1
- OTTHUMP00000013879
|
| PurificationMethod | AffinityPurfied |
| UniProtNumber | |
| UniProtSummary | FUNCTION:RasproteinsbindGDP/GTPandpossessintrinsicGTPaseactivity.
ENZYMEREGULATION:AlternatebetweenaninactiveformboundtoGDPandanactiveformboundtoGTP.Activatedbyaguaninenucleotide-exchangefactor(GEF)andinactivatedbyaGTPase-activatingprotein(GAP).
SUBCELLULARLOCATION:Cellmembrane;Lipid-anchor;Cytoplasmicside.Golgiapparatusmembrane;Lipid-anchor.Note=ShuttlesbetweentheplasmamembraneandtheGolgiapparatus.
PTM:PalmitoylatedbytheZDHHC9-GOLGA7complex.Acontinuouscycleofde-andre-palmitoylationregulatesrapidexchangebetweenplasmamembraneandGolgi.
INVOLVEMENTINDISEASE:DefectsinNRASareacauseofjuvenilemyelomonocyticleukemia(JMML)[MIM:607785].JMMLisapediatricmyelodysplasticsyndromethatconstitutesapproximately30%ofchildhoodcasesofmyelodysplasticsyndrome(MDS)and2%ofleukemia.
MutationswhichchangeAA12,13or61activatethepotentialofRastotransformculturedcellsandareimplicatedinavarietyofhumantumors.
SEQUENCESIMILARITIES:BelongstothesmallGTPasesuperfamily.Rasfamily.
|
| MolecularWeight | ~48kDa(Speculatedtohavespecificityfordimerizedform) |