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    當前位置 ： Millipore >>> Millipore/07-596 | Anti-Histone H4 (citrulline 3) Antibody/07-596/200 µL
    Millipore/07-596 | Anti-Histone H4 (citrulline 3) Antibody/07-596/200 µL
    • Millipore/07-596 | Anti-Histone H4 (citrulline 3) Antibody/07-596/200 µL

    Millipore/07-596 | Anti-Histone H4 (citrulline 3) Antibody/07-596/200 µL

    價格: ￥3576.00 市場價: 5960.00
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    貨號: 07-596
    品牌: Millipore
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      • Description
        CatalogueNumber07-596
        BrandFamilyUpstate
        TradeName
        • Upstate
        DescriptionAnti-HistoneH4(citrulline3)Antibody
        AlternateNames
        • Cit-H4R3
        • H4Cit3
        • HistoneH4,Arg3citrullinated
        BackgroundInformationHistoneH4(UniProtP62805;alsoknownasH4histonefamilymemberA,Histone1H4a)isencodedbytheHIST1H4A(alsoknownasH4/A,H4FA)gene(GeneID8359)inhuman.HistoneH4isoneofthemainhistoneproteinsthatcontributetothechromatinstructureinbothprokaryoticandeukaryoticcells.HistoneH4canundergoseveraldifferenttypesofepigeneticmodificationsthatinfluencecellularprocesses,includingmethylationandacetylationonlysineandarginineresidues,phosphorylationonserineandthreonineresidues,aswellasconversionArg3toacitrullineresiduebythepeptidylargininedeiminasePAD4-catalyzeddeiminationreaction,alsoknownascitrullination).HistoneH4Arg3citrullination(H4Cit3)weakenshistone-DNAinteractionasaresultofaconformationalchangeandareducedpositivechargeatthehistoneH4Nterminalend.BacterialinfectionisknowntostimulateH4Cit3inneutrophils,forminghighlydecondensedextracellularchromatinstructureNET(neutrophilextracellulartrap)thatcouldcaptureandkillmicroorganisms.Ontheotherhand,citrullinatedproteinsarerecognizedasnon-selfproteinsandsubsequentlyactivateimmunesystems.AfunctionalhaplotypeofthePADI4genehasbeenshowntobeassociatedwiththepathogenesisofrheumatoidarthritisthroughinductionoftheantigenicityofproteins.H4Cit3isinducedinresponsetoDNAdamagethroughthep53-PADI4pathway,resultinginupregulatedH4Cit3immunoreactivitylocalizedaroundfragmentednucleiinapoptoticcells.Inaddition,H4Cit3levelisnegativelycorrelatedwithp53proteinexpressionandtumoursizeinnon-smallcelllungcancer(NSCLC)tissues,indicatingacrucialroleofH4Cit3incarcinogenesis.
        ProductInformation
        FormatAffinityPurified
        PresentationPurifiedrabbitpolyclonalantibodyinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazide.
        StorageandShippingInformation
        StorageConditionsStablefor1yearat2-8°Cfromdateofreceipt.
        Applications
        ApplicationAnti-HistoneH4(Citrulline3)Antibody,Cat.No.07-596,isahighlyspecificrabbitpolyclonalantibodythattargetshistoneH4citrullination(H4Cit3)andhasbeentestedinChromatinImmunoprecipitation(ChIP),Immunocytochemistry,Immunohistochemistry,andWesternBlotting.
        KeyApplications
        • ChromatinImmunoprecipitation(ChIP)
        • Immunocytochemistry
        • Immunohistochemistry(Paraffin)
        • WesternBlotting
        ApplicationNotesChromatinImmunoprecipitation(ChIP)Analysis:Arepresentativelot(3μLperChIP)detectedhistoneH4Arg3citrullination(H4Cit3)enrichmentatthepS2genepromoterinMCF-7cellsthathadbeentreatedwithβ-estradiolforonehouraftera3-dayserum-starvationperiod.

        ImmunocytochemistryAnalysis:A1:500dilutiondetectedfromarepresentativelotdetectedenhancedhistoneH4Arg3citrullinationinionomycin-stimulatedprimaryhumanneutrophils(CourtesyofKerriMowen,Ph.D.,PadlockTherapeutics).

        ImmunohistochemistryAnalysis:A1:250dilutionfromarepresentativelotdetectedenhancedhistoneH4Arg3citrullination(H4Cit3)in4%paraformaldehyde-fixed,decalcified,paraffin-embeddedhindpawsectionsfromwild-type,butnotPad4-knockoutmice5dayspostimmunizationwitharthritogenicserumfromK/BxNmice(CourtesyofKerriMowen,Ph.D.,PadlockTherapeutics).

        ChromatinImmunoprecipitation(ChIP)Analysis:Arepresentativelotdetectedatime-dependenthistoneH4Arg3citrullination(H4Cit3)enrichmentatthepS2genepromoterinMCF-7cellsfollowingestradioltreatment(Wang,Y.,etal.(2004).Science.306(5694):279-283).

        ImmunocytochemistryAnalysis:ArepresentativelotdetectedenhancednuclearH4/H2AAr3citrullinationinHEK293TandU2OScellsuponexogenousPAD4expressionorDNAdamageinduction(Tanikawa,C.,etal.(2012).Nat.Commun.3:676).

        ImmunocytochemistryAnalysis:ArepresentativelotdetectedArg3-citrullinatedhistoneH4(H4Cit3)in3.8%paraformaldehyde-fixed,2%NP-40/1%TritonX-100-permeABIlizedcasein-elicitedperitonealneutrophilscellsfromwild-type,butnotPad4-knockoutmice(Hemmers,S.,etal.(2011).PLoSOne.6(7):e22043).

        ImmunocytochemistryAnalysis:ArepresentativelotdetectedArg3-citrullinatedhistoneH4(H4Cit3)in3.8%paraformaldehyde-fixed,2%NP-40/1%TritonX-100-permeabilizedinfiltratinglungleukocytesisolatedfromwild-type,butnotPad4-knockoutmicefollowinginfluenzainfection(Hemmers,S.,etal.(2011).PLoSOne.6(7):e22043).

        ImmunocytochemistryAnalysis:ArepresentativelotdetectedArg3-citrullinatedhistoneH4(H4Cit3)in4%paraformaldehyde-fixed,1%NP-40/0.5%TritonX-100-permeabilizedperipheralbloodcellsfromwild-type,butnotPad4-knockoutmice5dayspostimmunizationwitharthritogenicserumfromK/BxNmice(Rohrbach,A.S.,etal.(2012).ArthritisRes.Ther.14(3):R104).

        ImmunocytochemistryAnalysis:ArepresentativelotdetectedArg3-citrullinatedhistoneH4(H4Cit3)insynovialcellsandextracellulartraps(NETs)flushedoutoftheanklejointsofwild-type,butnotPad4-knockoutmice5dayspostimmunizationwitharthritogenicserumfromK/BxNmice(Rohrbach,A.S.,etal.(2012).ArthritisRes.Ther.14(3):R104).

        ImmunocytochemistryAnalysis:Arepresentativelotdetectedcalciumionophore-inducedhistoneH4(H4Cit3)Arg3citrullinationin3.7%paraformaldehyde-fixed,0.2%NP-40/0.1%TritonX-100-permeabilizedHL-60cells(Wang,Y.,etal.(2004).Science.306(5694):279-283).
        ImmunohistochemistryAnalysis:Arepresentativelotdetectedgammairradiation-inducednuclearH4/H2AAr3citrullinationinthymustissuesectionsfromwild-type,butnotPad4-knockoutmice(Tanikawa,C.,etal.(2012).Nat.Commun.3:676).

        ImmunohistochemistryAnalysis:ArepresentativelotdetectedenhancedhistoneH4Arg3citrullination(H4Cit3)in4%paraformaldehyde-fixed,decalcified,paraffin-embeddedhindpawsectionsfromwild-type,butnotPad4-knockoutmice10dayspostimmunizationwitharthritogenicserumfromK/BxNmice(Rohrbach,A.S.,etal.(2012).ArthritisRes.Ther.14(3):R104).

        WesternBlottingAnalysis:ArepresentativelotdetectedPAD4-catalyzedArg3citrullinationofrecombinanthistoneH4andH2A,aswellasDNAdamage-inducedcellularH4andH2AAr3citrullination(Tanikawa,C.,etal.(2012).Nat.Commun.3:676).

        WesternBlottingAnalysis:Arepresentativelotdetectedcalciumionophore-inducedhistoneH4Arg3citrullination(H4Cit3)inHL-60cells.CellularPAD4knockdownbyshRNAtreatmentgreatlydiminishedionophor-inducedH4Cit3,whileantibodyblockingwiththeimmunogenpeptidepreventedtargetbanddetection(Wang,Y.,etal.(2004).Science.306(5694):279-283).
        BIOLOGicalInformation
        ImmunogenKLH-conjugated,syntheticpeptidecorrespondingtoaminoacids1-10(SG[cit]GKGGKGL-C)ofhumanhistoneH4(Met1removed),where[cit]denotescitrullinatedArg3.AC-terminalcysteinewasaddedforconjugationpurposes.
        EpitopeN-terminus
        ConcentrationPleaserefertolotspecificdatasheet.
        HostRabbit
        SpecificityThisrabbitpolyclonalantibodydetectedPAD4-catalyzedArg3citrullinationofrecombinanthistoneH4andH2A,aswellascalciumionophore-inducedhistoneH4Arg3citrullination(H4Cit3)inHL-60cells.CellularPAD4(peptidylargininedeiminase4)knockdowngreatlydiminishedionophor-inducedH4Cit3,whileantibodyblockingwiththeimmunogenpeptidepreventedtargetbanddetection(Tanikawa,C.,etal.(2012).Nat.Commun.3:676;Wang,Y.,etal.(2004).Science.306(5694):279-283).
        SpeciesReactivity
        • Human
        • Mouse
        SpeciesReactivityNoteHuman,Mouse.Predictedtoreactwithabroadrangeofspeciesbasedon100%sequencehomology.
        AntibodyTypePolyclonalAntibody
        EntrezGeneNumber
        GeneSymbol
        • HIST1H4A
        • H4/A
        • ;H4FA
        • HIST1H4B
        • H4/I
        • ;H4FI
        • HIST1H4C
        • H4/G
        • H4FG
        • HIST1H4D
        • H4/B
        • H4FB
        • HIST1H4E
        • H4/J
        • H4FJ
        • HIST1H4F
        • H4/C
        • H4FC
        • HIST1H4H
        • H4/H
        • H4FH
        • HIST1H4I
        • H4/M
        • H4FM
        • HIST1H4J
        • H4/E
        • H4FE
        • HIST1H4K
        • H4/D
        • H4FD
        • HIST1H4L
        • H4/K
        • H4FK
        • HIST2H4A
        • H4/N
        • H4F2
        • H4FN
        • HIST2H4
        • HIST2H4B
        • H4/O
        • H4FO
        • HIST4H4
        • HIST1H4A
        • H4/A
        Modifications
        • Citrullination
        PurificationMethodAffinitypurified.
        UniProtNumber
        MolecularWeight~12kDaobserved.11.24/11.37kDa(H4with/withoutMet1removal)calculated.Uncharacterizedbandsmaybeobservedinsomelysate(s).
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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