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    當前位置 : Millipore >>> Millipore/07-1413 | Anti-GSK-3b Antibody, a.a. 335-349/07-1413/50 µg
    Millipore/07-1413 | Anti-GSK-3b Antibody, a.a. 335-349/07-1413/50 µg
    • Millipore/07-1413 | Anti-GSK-3b Antibody, a.a. 335-349/07-1413/50 µg

    Millipore/07-1413 | Anti-GSK-3b Antibody, a.a. 335-349/07-1413/50 µg

    價格: ¥3696.00 市場價: 6160.00

    貨號: 07-1413
    品牌: Millipore
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      • Description
        CatalogueNumber07-1413
        ReplacesAB8687
        DescriptionAnti-GSK-3bAntibody,a.a.335-349
        BackgroundInformationGSK3(GlycogenSynthaseKinase3)differsfrommostserine/threoninekinasesinthatitisactiveintheabsenceoftheactionofsignalingpathways.Twoisoformsexist,GSK3αandGSK3β.ThefunctionofGSK3istophosphorylateGlycogenSynthaseandtherebyinactivateit.InsulinactionstimulatesthePI3Kinasepathway,resultinginAktactivation,whichphosphorylatesandinactivatesGSK3.GlycogenSynthaseisthenrapidlydephosphorylatedandactivated.OtherGSK3substratesincludeJun(oninhibitorysites),andeIF2B.PhosphorylationofTaubyGSK3mayrelatetodevelopmentofAlzheimer"sdisease.Phosphorylationstate-specificantibodiesdirectedagainsttheAktsite(Ser21)onGSK3aresuitableforsurrogateassaysoftheactivationstateofthepathway.
        ProductInformation
        FormatAffinityPurified
        Control
        • C2C12CellLysate,Helacells,ratormousebrain
        PresentationPurifiedpolyclonalIgGantibodyissuppliedinPBS,pH7.2,50%glyceroland0.09%sodiumazide.
        StorageandShippingInformation
        StorageConditionsStableat–20oCforuptooneyearfromdateofreceipt.Productmaybestoredatfor2-4weeksat2o-8oC.
        HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.
        Applications
        ApplicationThisAntiI-GSK-3bAntibody,a.a.335-349isvalidatedforuseinWB,IP,IH(P)forthedetectionofAntiI-GSK-3b.
        KeyApplications
        • WesternBlotting
        • Immunoprecipitation
        • Immunohistochemistry(Paraffin)
        ApplicationNotesImmunoprecipitation:12.5μg/mLdilutionofapreviouslotwasuedinimmunoprecipitationonratbraintissue;immunoprecipitationswithtissueextractsotherthanbrainmayrequirehigherantibodyconcentrations.
        Optimalworkingdilutionsmustbedeterminedbytheenduser.

        Immunohistochemistry(paraffin):
        Representativetestingfromapreviouslot.
        OptimalStainingofGSK3βPolyclonalAntibody:HeLaCells
        BIOLOGicalInformation
        ImmunogenRatGSK3betasyntheticpeptide(aa.335-349),conjugatedtoKLH.Thissequenceis100%identicaltohumanGSK3beta.
        Epitopea.a.335-349
        ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
        HostRabbit
        SpecificityThisantibodydetectsastrong47kDaprotein,correspondingtotheexpectedmolecularmassofGSK3beta.
        IsotypeIgG
        SpeciesReactivity
        • Rat
        • Human
        • Mouse
        • Bovine
        AntibodyTypePolyclonalAntibody
        EntrezGeneNumber
        EntrezGeneSummaryGlycogensynthasekinase-3(GSK3)isaproline-directedserine-threoninekinasethatwasinitiallyidentifiedasaphosphorylatingandnactivatingglycogensynthase.Twoisoforms,alpha(GSK3A;MIM606784)andbeta,showahighdegreeofaminoacidhomology(StambolicandWoodgett,1994[PubMed7980435]).GSK3Bisinvolvedinenergymetabolism,neuronalcelldevelopment,andbodypatternformation(Plyteetal.,1992[PubMed1333807]).[suppliedbyOMIM].
        GeneSymbol
        • GSK3B
        • EC2.7.11.26
        PurificationMethodProteinAaffinitypurified
        UniProtNumber
        UniProtSummaryFUNCTION:ParticipatesintheWntsignalingpathway.Implicatedinthehormonalcontrolofseveralregulatoryproteinsincludingglycogensynthase,MYBandthetranscriptionfactorJUN.PhosphorylatesJUNatsitesproximaltoitsDNA-bindingdomain,therebyreducingitsaffinityforDNA.PhosphorylatesMUC1inbreastcancercells,anddecreasestheinteractionofMUC1withCTNNB1/beta-catenin.
        CATALYTICACTIVITY:ATP+[tauprotein]=ADP+[tauprotein]phosphate.
        ENZYMEREGULATION:InhibitedwhenphosphorylatedbyAKT1.
        SUBUNIT:Monomer(Bysimilarity).InteractswithCABYR,MUC1,NINandPRUNE.
        ALTERNATIVEPRODUCTS:2namedisoforms[FASTA]producedbyalternativesplicing.
        MolecularWeightThisantibodydetectsastrong47kDaprotein.
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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