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    當前位置 : Millipore >>> Millipore/06-847 | Anti-EGFR Antibody/06-847/200 µg
    Millipore/06-847 | Anti-EGFR Antibody/06-847/200 µg
    • Millipore/06-847 | Anti-EGFR Antibody/06-847/200 µg

    Millipore/06-847 | Anti-EGFR Antibody/06-847/200 µg

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    貨號: 06-847
    品牌: Millipore
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      • Description
        CatalogueNumber06-847
        Replaces04-337;04-338
        BrandFamilyUpstate
        TradeName
        • Upstate
        DescriptionAnti-EGFRAntibody
        AlternateNames
        • Receptortyrosine-proteinkinaseErbB-1
        • avianerythroblasticleukemiaviral(v-erb-b)oncogenehomolog
        • cellgrowthinhibitingprotein40
        • cellproliferation-inducingprotein61
        • epidermalgrowthfactorreceptor
        • epidermalgrowthfactorreceptor(avianerythroblasticleukemiaviral
          (v-erb-b)oncogenehomolog)
        • epidermalgrowthfactorreceptor(erythroblasticleukemiaviral(v-erb-b)oncogenehomolog,avian)
        BackgroundInformationTheepidermalgrowthfactorreceptor(EGFR;ErbB-1;HER1inhumans)isthecell-surfacereceptorformembersoftheepidermalgrowthfactorfamily(EGF-family)ofextracellularproteinligands.TheepidermalgrowthfactorreceptorisamemberoftheErbBfamilyofreceptors,asubfamilyoffourcloselyrelatedreceptortyrosinekinases:EGFR(ErbB-1),HER2/c-neu(ErbB-2),Her3(ErbB-3)andHer4(ErbB-4).MutationsaffectingEGFRexpressionoractivitycouldresultincancer.EGFR(epidermalgrowthfactorreceptor)existsonthecellsurfaceandisactivatedbybindingofitsspecificligands,includingepidermalgrowthfactorandtransforminggrowthfactorα(TGFα).Uponactivationbyitsgrowthfactorligands,EGFRundergoesatransitionfromaninactivemonomericformtoanactivehomodimer.Inadditiontoforminghomodimersafterligandbinding,EGFRmaypairwithanothermemberoftheErbBreceptorfamily,suchasErbB2/Her2/neu,tocreateanactivatedheterodimer.EGFRdimerizationstimulatesitsintrinsicintracellularprotein-tyrosinekinaseactivity.Asaresult,autophosphorylationoffivetyrosine(Y)residuesintheC-terminaldomainofEGFRoccurs.
        ProductInformation
        FormatPurified
        Control
        • A431celllysate,humancervicalcarcinoma.

          IncludedPositiveAntigenControl:
          Catalog#12-305,3T3/A31CellLysate.Add2.5μLof2-mercaptoethanol/100μLoflysateandboilfor5minutestoreducethepreparation.Load20μgofreducedlysateperlaneforminigels.
        PresentationPurifiedrabbitpolyclonalIgGinbuffercontaining0.1MTris-Glycine,0.15MNaCl,0.05%SodiumAzide,pH7.4.Storeat2-8°C.
        StorageandShippingInformation
        StorageConditionsStablefor1yearat2-8°Cfromdateofreceipt.

        HandlingRecommendations:Uponfirstthaw,
        andpriortoremovingthecap,centrifugethe
        vialandgentlymixthesolution.
        Applications
        ApplicationAnti-EGFRAntibodydetectslevelofEGFR&hasbeenpublished&validatedforuseinIP&WB.
        KeyApplications
        • Immunoprecipitation
        • WesternBlotting
        ApplicationNotesImmunoprecipitation:
        4μgofapreviouslotimmunoprecipitatedtheEGFRfrom500μgofa3T3/A31RIPAcelllysate.
        BIOLOGicalInformation
        ImmunogenOvalbuminconjugatedsyntheticpeptide(ETKPNGIFKGPTAENAEYLRVAPPSSEFIGA)correspondingtoaminoacids1156-1186oftheprocessedmouseEGFreceptor(EGFR)C-terminaldomain(AccessionnumberQ01279).Theimmunizingsequenceshares28/31identitywiththehumanEGFreceptorsequence.
        ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
        HostRabbit
        SpecificityRecognizestheEGFR,Mr180kDa.
        IsotypeIgG
        SpeciesReactivity
        • Human
        • Mouse
        • Rat
        • Hamster
        SpeciesReactivityNoteMouseandhuman.Reportedtodetectratandhamster.
        AntibodyTypePolyclonalAntibody
        EntrezGeneNumber
        EntrezGeneSummaryTheproteinencodedbythisgeneisatransmembraneglycoproteinthatisamemberoftheproteinkinasesuperfamily.Thisproteinisareceptorformembersoftheepidermalgrowthfactorfamily.EGFRisacellsurfaceproteinthatbindstoepidermalgrowthfactor.Bindingoftheproteintoaligandinducesreceptordimerizationandtyrosineautophosphorylationandleadstocellproliferation.Mutationsinthisgeneareassociatedwithlungcancer.[providedbyRefSeq]
        GeneSymbol
        • EGFR
        • ERBB1
        • ERBB
        • mENA
        PurificationMethodProteinAchromatography
        UniProtNumber
        UniProtSummaryFUNCTION:SwissProt:P00533#Isoform2/truncatedisoformmayactasanantagoNIST.
        SIZE:1210aminoacids;134277Da
        SUBUNIT:BindsRIPK1.CBLinteractswiththeautophosphorylatedC-terminaltailoftheEGFreceptor.PartofacomplexwithERBB2andeitherPIK3C2AorPIK3C2B.TheautophosphorylatedforminteractswithPIK3C2B,maybeindirectly.InteractswithPELP1.
        SUBCELLULARLOCATION:Cellmembrane;Single-passtypeImembraneprotein.&Isoform2:Secreted.
        TISSUESPECIFICITY:Expressedinplacenta.Isoform2isalsoexpressedinovariancancers.
        PTM:PhosphorylationofSer-695ispartialandoccursonlyifThr-693isphosphorylated.&MonoubiquitinatedandpolyubiquitinateduponEGFstimulation;whichdoesnotaffecttyrosinekinaseactivityorsignalingcapacitybutmayplayaroleinlysosomaltargeting.Polyubiquitinlinkageismainlythrough"Lys-63",butlinkagethrough"Lys-48","Lys-11"and"Lys-29"alsooccur.DISEASE:SwissProt:P00533#DefectsinEGFRareassociatedwithlungcancer[MIM:211980].
        SIMILARITY:SwissProt:P00533##Belongstotheproteinkinasesuperfamily.Tyrproteinkinasefamily.EGFreceptorsubfamily.&Contains1proteinkinasedomain.
        MISCELLANEOUS:BindingofEGFtothereceptorleadstodimerization,internalizationoftheEGF-receptorcomplex,inductionofthetyrosinekinaseactivity,stimulationofcellDNAsynthesis,andcellproliferation.
        MolecularWeight180kDa
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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