| Immunogen | KLH-conjugated,syntheticpeptidecorrespondingtoaminoacidresidues129-149ofhumanCaspase1(PEHKTSDSTFLVFMSHGIREG). |
| Epitope | a.a.129-149 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Rabbit |
| Specificity | Recognizesthep45proenzyme,Mr45kDa,andthep20subunit,Mr20kDa,ofreducedhumanCaspase1isoforms. |
| Isotype | IgG |
| SpeciesReactivity | |
| SpeciesReactivityNote | Human,Mouse,Rat.Reactivitywithotherspecieshasnotbeenconfirmed. |
| AntibodyType | PolyclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Thisgeneencodesaproteinwhichisamemberofthecysteine-asparticacidprotease(caspase)family.Sequentialactivationofcaspasesplaysacentralroleintheexecution-phaseofcellapoptosis.Caspasesexistasinactiveproenzymeswhichundergoproteolyticprocessingatconservedasparticresiduestoproduce2subunits,largeandsmall,thatdimerizetoformtheactiveenzyme.Thisgenewasidentifiedbyitsabilitytoproteolyticallycleaveandactivatetheinactiveprecursorofinterleukin-1,acytokineinvolvedintheprocessessuchasinflammation,septicshock,andwoundhealing.Thisgenehasbeenshowntoinducecellapoptosisandmayfunctioninvariousdevelopmentalstages.StudiesofasimilargeneinmousesuggestaroleinthepathogenesisofHuntingtondisease.Alternativesplicingofthisgeneresultsinfivetranscriptvariantsencodingdistinctisoforms. |
| GeneSymbol | - CASP1
- IL1BCE
- P45
- IL-1BC
- IL1BC
- p45
- IL1B-convertase
- CASP-1
- ICE
|
| PurificationMethod | ProteinAPurfied |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P29466#ThiolproteasethatcleavesIL-1betabetweenanAspandanAla,releasingthematurecytokinewhichisinvolvedinavarietyofinflammatoryprocesses.Importantfordefenseagainstpathogens.Cleavesandactivatessterolregulatoryelementbindingproteins(SREBPs).Canalsopromoteapoptosis. SIZE:404aminoacids;45159Da SUBUNIT:Heterotetramerthatconsistsoftwoanti-parallelarrangedheterodimers,eachoneformedbya20kDa(p20)anda10kDa(p10)subunit.Thep20subunitcanalsoformaheterodimerwiththeepsilonisoformwhichthenhasaninhibitoryeffect.Maybeacomponentoftheinflammasome,aproteincomplexwhichalsoincludesPYCARD,CARD8andNALP2andwhosefunctionwouldbetheactivationofproinflammatorycaspases. SUBCELLULARLOCATION:Cytoplasm. TISSUESPECIFICITY:Expressedinlargeramountsinspleenandlung.Detectedinliver,heart,smallintestine,colon,thymus,prostate,skeletalmuscle,peripheralbloodleukocytes,kidneyandtestis.Noexpressioninthebrain. PTM:Thetwosubunitsarederivedfromtheprecursorsequencebyanautocatalyticmechanism. SIMILARITY:SwissProt:P29466##BelongstothepeptidaseC14family.&Contains1CARDdomain....hide?seemore? |
| MolecularWeight | Proenzyme(45kDa)andp20subunit(20kDa) |