• <menu id="uuusw"></menu>
    •
  • <table id="uuusw"></table>
    當前位置 ： Millipore >>> Millipore/05-806 | Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10/05-806/100 µg
    Millipore/05-806 | Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10/05-806/100 µg
    • Millipore/05-806 | Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10/05-806/100 µg

    Millipore/05-806 | Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10/05-806/100 µg

    價格: 試用 市場價: 0.00
    打印產品信息
    貨號: 05-806
    品牌: Millipore
    規格
    數量
    庫存(0)
    特別 提示
    代購產品：無質量問題不接受退換貨，下單前請仔細核對信息。
    下單后請及時聯系客服核對商品價格，訂單生效后再付款。
    立即申請

    收藏商品
    資深產品顧問
    咨詢顧問

    全國免費服務熱線

    4000-520-616

    • 自營商城 一站式服務
    • 廠家直采 剔除溢價
    • 品質甄選 正品保證
    • 嚴控流程 只做188精品
    • 極速物流 如約送貨
    • 詳情
    • 使用說明
    • 常見問題
      • Description
        CatalogueNumber05-806
        BrandFamilyUpstate
        TradeName
        • Upstate
        DescriptionAnti-phospho-HistoneH3(Ser10)Antibody,clone3H10
        AlternateNames
        • H3histone,family3A
        • H3S10P
        • HistoneH3(phosphoS10)
        • H3histone,family3A
        • H3histone,family3B
        • H3histone,family3B(H3.3B)
        BackgroundInformationHistoneH3isoneofthefivemainhistoneproteinsinvolvedinthestructureofchromatinineukaryoticcells.FeaturingamainglobulardomainandalongN-terminaltail,H3isinvolvedwiththestructureofthenucleosomesofthe"beadsonastring"structure.TheN-terminaltailofhistoneH3protrudesfromtheglobularnucleosomecoreandcanundergoseveraldifferenttypesofepigeneticmodificationsthatinfluencecellularprocesses.Thesemodificationsincludethecovalentattachmentofmethyloracetylgroupstolysineandarginineaminoacidsandthephosphorylationofserineorthreonine.
        ProductInformation
        FormatPurified
        Control
        • UV-treated293cellextracts,UV-treatedHeLacellextracts,breastcancertissue,HEPG2cellextracts.
        PresentationPurifiedmouseIgG1kinbuffercontaining0.1MTris-glycine,pH7.4,0.15MNaCland0.05%sodiumazide.
        StorageandShippingInformation
        StorageConditionsStablefor1yearat2to8°Cfromdateofreceipt.

        HandlingRecommendations:
        Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.
        Applications
        ApplicationAnti-phospho-HistoneH3(Ser10)Antibody,clone3H10isamousemonoclonalantibodyfordetectionofHistoneH3phosphorylatedatserine10.ThispurifiedmAb,alsoknownasH3S10p,ispublishedinpeerreviewedjournalsandhasbeenvalidatedinFC,ICC,IF,PIA,WB,Mplex.
        KeyApplications
        • FlowCytometry
        • Immunocytochemistry
        • Immunofluorescence
        • PeptideInhibitionAssay
        • WesternBlotting
        • Multiplexing
        ApplicationNotesFlowCytometry:
        ThisantibodyhasbeenreportedbyanindependentlaboratorytodetectphosphorylatedhistoneH3usingflowcytometry.

        Beadlyte?Histone-PeptideSpecificityAssay:
        1:1,000to1:81,000dilutionsofapreviouslotwereincubatedwithhistoneH3peptidescontainingvariousmodificationsconjugatedtoLuminex?microspheres.(FigureB).Onlythepeptidecontainingphospho-serine10wasdetected.


        Immunocytochemistry:
        0.2μg/mLofapreviouslotshowedpositivechromosomeimmunostainingformitoticA431andHeLacellsfixedwith95%ethanoland5%aceticacidandpermeabilizedwith0.1%Triton?X-100.

        PeptideInhibitionAnalysis:
        DetectionofhistoneH3inimmunoblotsofcolcemidtreatedHeLaacidextractsbyanti-phospho-HistoneH3(Ser10)wasdiminishedby10μMofhistoneH3peptidecontainingphospho-serine10,butnotbypeptidescontainingphospho-serine28oranunmodifiedhistoneH3sequence(FigureC).

        Immunofluorescence:
        BIOLOGicalInformation
        ImmunogenAproprietaryimmunogenbasedonapeptidesequencecontainingphospho-serinecorrespondingtoresidue10ofhumanhistoneH3.
        Clone3H10
        ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
        HostMouse
        SpecificityHistoneH3phosphorylatedatSer10.
        IsotypeIgG1κ
        SpeciesReactivity
        • Human
        SpeciesReactivityNoteHuman.
        Broadspeciescross-reactivityisexpected.
        AntibodyTypeMonoclonalAntibody
        EntrezGeneNumber
        EntrezGeneSummaryHistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Nucleosomesconsistofapproximately146bpofDNAwrappedaroundahistoneoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.ThisgeneisintronlessandencodesamemberofthehistoneH3family.TranscriptsfromthisgenelackpolyAtails;instead,theycontainapalindromicterminationelement.ThisgeneislocatedseparatelyfromtheotherH3genesthatareinthehistonegeneclusteronchromosome6p22-p21.3.
        GeneSymbol
        • HIST1H3A
        • H3FA
        • HIST1H3B
        • H3FL
        • HIST1H3C
        • H3FC
        • HIST1H3D
        • H3FB
        • HIST1H3E
        • H3FD
        • HIST1H3F
        • H3FI
        • HIST1H3G
        • H3FH
        • HIST1H3H
        • H3FK
        • HIST1H3I
        • H3FF
        • HIST1H3J
        • H3FJ
        • HIST3H3
        • H3FT
        • H3T
        • MGC126886
        Modifications
        • Phosphorylation
        PurificationMethodProteinGPurified
        UniProtNumber
        UniProtSummaryFUNCTION:SwissProt:Q16695#Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstability.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.
        SIZE:136aminoacids;15508Da
        SUBUNIT:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.
        SUBCELLULARLOCATION:Nucleus.
        TISSUESPECIFICITY:Expressedintesticularcells.
        DEVELOPMENTALSTAGE:ExpressedduringSphase,thenexpressionstronglydecreasesascelldivisionslowsdownduringtheprocessofdifferentiation.
        PTM:Acetylationisgenerallylinkedtogeneactivation.AcetylationonLys-10impairsmethylationatArg-9.AcetylationonLys-19andLys-24favorsmethylationatArg-18(Bysimilarity).&CitrullinationatArg-9and/orArg-18byPADI4impairsmethylationandrepressestranscription(Bysimilarity).&AsymmetricdimethylationatArg-18byCARM1islinkedtogeneactivation.SymmetricdimethylationatArg-9byPRMT5islinkedtogenerepression(Bysimilarity).&MethylationatLys-5,Lys-37andLys-80arelinkedtogeneactivation.MethylationatLys-5facilitatessubsequentacetylationofH3andH4.MethylationatLys-80isassociatedwithDNAdouble-strandbreak(DSB)responsesandisaspecifictargetforTP53BP1.MethylationatLys-10andLys-28arelinkedtogenerepression.MethylationatLys-10isaspecifictargetforHP1proteins(CBX1,CBX3andCBX5)andpreventssubsequentphosphorylationatSer-11andacetylationofH3andH4.MethylationatLys-5andLys-80requirepreliminarymonoubiquitinationofH2Bat"Lys-120".MethylationatLys-10andLys-28areenrichedininactiveXchromosomechromatin(Bysimilarity).&PhosphorylatedatThr-4byGSG2/haspinduringprophaseanddephosphorylatedduringanaphase.Atcentromeres,specificallyphosphorylatedatThr-12fromprophasetoearlyanaphase.PhosphorylatedatSer-11duringthewholemitosis.PhosphorylationatSer-11,whichislinkedtogeneactivation,preventsmethylationatLys-10butfacilitatesacetylationofH3andH4.PhosphorylatedatSer-29byMLTKisoform1,RPS6KA5orAURKBduringmitosisoruponultravioletBirradiation(Bysimilarity).&Phosphorylationat"Ser-11"iscrucialforchromosomecondensationandcell-cycleprogressionduringmitosisandmeiosis.Inadditionphosphorylationat"Ser-11"isimportantduringinterphasebecauseitenablesthetranscriptionofgenesfollowingexternalstimulation,likestressorgrowthfactors.Phosphorylationat"Ser-11"isalsoanessentialregulatorymechanismforneoplasticcelltransformation.Phosphorylationat"Ser-11"byAURKB/Aurora-BmediatesthedissociationofHP1proteins(CBX1,CBX3andCBX5)fromheterochromatin.&Ubiquitinated(Bysimilarity).
        SIMILARITY:SwissProt:Q16695##BelongstothehistoneH3family.
        MolecularWeight17kDa
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
      售后保障
      螞蟻淘生物188，秉承螞蟻淘一貫的嚴謹態度，由螞蟻淘公司專業人員負責品控、采購、物流、銷售、售后，保障正品優質。以“快速好省，為科研提供好產品、好價格”為理念，直接鏈接原廠家，從全國各地原制造商嚴格挑選188款科研精品，剔除品牌溢價，188生物新電商，把好的產品帶給科研！? 力求給你最優質的商品。
    • Q：生物188產品正品保障嗎？
      A：生物188質量把控人員具有十年的從業經驗，在業界享有良好的口碑；自營商城，直接從廠家采購， 自己的團隊負責國際物流和清關，中間沒有第三方，所有流程嚴格把控，100%保證正品，假一罰十。

      Q：下單后可以修改訂單嗎？
      A：下單后的商品付款之前可以修改；訂單付款成功，需要聯系我們客服進行修改；客服電話：4000-520-616

      Q：可以開發票嗎？
      A：本網站所售商品都是正規清關，均開具16%正規發票，發票金額含配送費金額，另有說明的除外。

      Q：商品幾天可以發貨？
      A：生物188商品，全部現貨銷售，付款后即可發貨,一般一周內送達！

      Q：如何聯系商家？
      A：有任何問題夠可以電話咨詢我們，全國24小時免費服務熱線：4000-520-616 或聯系我們的在線客服QQ：1570468124

      Q：收到的商品少了/發錯了怎么辦？
      A：同個訂單購買多個商品可能會分為一個以上包裹發出，可能不會同時送達，建議查看訂單詳情是否是 部分發貨狀態；如未收到，可聯系在線客服或者致電4000-520-616。

      Q：退換貨/維修需要多長時間？
      A：一般情況下，退貨處理周期為客戶收到產品一個月內（以快遞公司顯示簽收時間為準），包裝規格、 數量、品種不符，外觀毀損、短缺或缺陷，請在收到貨24小時內申請退換貨；特殊商品以合同條款為準。

    客戶服務

    在線客服
    用戶反饋

    何為188

    極簡而嚴謹，我們僅銷售188款生物醫學科研用品，款款都是爆款；因為少所以聚焦，聚焦甄選每一款產品，聚焦服務每一位客戶!

    關注我們 :

    點擊QQ聯系我們
    生物188微信

    關注188微信公眾號

    獲取最新優惠活動通知

    • 品質甄選，正品保證

    • 自營電商，廠家直采

    • 極簡主義，188精品

    常見問題 | 購買相關 | 配送與驗收 | 支付問題 | 售后與服務 | 服務協議 | 隱私政策 | 何為188 | 中國智造 | 聯系我們
    友情鏈接： ImmunoChem中國 | Paragon Genomics價格 | phelpsd產品代理 | MagnaMedics廠家 | ezbiosystems代理 | 螞蟻淘 | 紅外干燥箱

    公司版權所有 ? 2005-2021 蘇州螞蟻淘生物科技有限公司 蘇ICP備17049038號-10

  • <menu id="uuusw"></menu>
    •
  • <table id="uuusw"></table>
    上床视频