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    當前位置 : Millipore >>> Millipore/05-710 | Anti-NG2 Antibody, clone 132.38/05-710/200 µg
    Millipore/05-710 | Anti-NG2 Antibody, clone 132.38/05-710/200 µg
    • Millipore/05-710 | Anti-NG2 Antibody, clone 132.38/05-710/200 µg

    Millipore/05-710 | Anti-NG2 Antibody, clone 132.38/05-710/200 µg

    價格: ¥4680.00 市場價: 7800.00

    貨號: 05-710
    品牌: Millipore
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      • Description
        CatalogueNumber05-710
        BrandFamilyUpstate
        TradeName
        • Upstate
        DescriptionAnti-NG2Antibody,clone132.38
        ProductInformation
        FormatPurified
        Control
        • Braintissue
        PresentationProteinAPurifiedimmunoglobulinin0.1MTris-glycine,pH7.4,0.15MNaClwith0.05%sodiumazideasapreservative.
        StorageandShippingInformation
        StorageConditionsMaintainfor2yearsat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
        Applications
        ApplicationAnti-NG2Antibody,clone132.38detectslevelofNG2&hasbeenpublished&validatedforuseinIH&WB.
        KeyApplications
        • Immunohistochemistry
        • WesternBlotting
        ApplicationNotesCross-reactivityexpectedwithmouse.
        BIOLOGicalInformation
        ImmunogenHEK293cellsexpressingatruncatedintegralmembraneformofNG2consistingofaminoacids1592-2222
        Clone132.38
        ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
        HostMouse
        SpecificityRecognizesNG2.
        IsotypeIgG1
        SpeciesReactivity
        • Mouse
        • Rat
        AntibodyTypeMonoclonalAntibody
        EntrezGeneNumber
        EntrezGeneSummaryAhumanmelanoma-associatedchondroitinsulfateproteoglycanplaysaroleinstABIlizingcell-substratuminteractionsduringearlyeventsofmelanomacellspreADIngonendothelialbasementmembranes.CSPG4representsanintegralmembranechondroitinsulfateproteoglycanexpressedbyhumanmalignantmelanomacells.
        GeneSymbol
        • CSPG4
        • MCSP
        • HMW-MAA
        • MCSPG
        • MSK16
        • NG2
        • MEL-CSPG
        PurificationMethodProteinGPurified
        UniProtNumber
        UniProtSummaryFUNCTION:SwissProt:Q6UVK1#Proteoglycanplayingaroleincellproliferationandmigrationwhichstimulatesendothelialcellsmotilityduringmicrovascularmorphogenesis.Mayalsoinhibitneuriteoutgrowthandgrowthconecollapseduringaxonregeneration.Cellsurfacereceptorforcollagenalpha2(VI)whichmayconfercellsabilitytomigrateonthatsubstrate.BindsthroughitsextracellularN-terminusgrowthfactors,extracellularmatrixproteasesmodulatingtheiractivity.MayregulateMPP16-dependentdegradationandinvasionoftypeIcollagenparticipatinginmelanomacellsinvasionproperties.Maymodulatetheplasminogensystembyenhancingplasminogenactivationandinhibitingangiostatin.FunctionsalsoasasignaltransducingproteinbybindingthroughitscytoplasmicC-terminusscaffoldingandsignalingproteins.MaypromoteretractionfiberformationandcellpolarizationthroughRhoGTPaseactivation.Maystimulatealpha-4,beta-1integrin-mediatedadhesionandspreadingbyrecruitingandactivatingasignalingcascadethroughCDC42,ACK1andBCAR1.MayactivateFAKandERK1/ERK2signalingcascades.


        SIZE:2322aminoacids;250497Da

        SUBUNIT:InteractswiththefirstPDZdomainofMPDZ.InteractswithPRKCA.BindsTNC,laminin-1,COL5A1andCOL6A2.InteractswithPLGandangiostatin.BindsFGF2andPDGFA.InteractswithGRIP1,GRIP2andGRIA2.FormsaternarycomplexwithGRIP1andGRIA2(Bysimilarity).InteractswithLGALS3andtheintegrincomposedofITGB1andITGA3.InteractswithITGA4throughitschondroitinsulfateglycosaminoglycan.InteractswithBCAR1,CDC42andACK1.InteractswithMMP16.

        SUBCELLULARLOCATION:Apicalcellmembrane;Single-passtypeImembraneprotein(Bysimilarity).Cellprojection,lamellipodium(Bysimilarity).Note=LocalizedattheapicalplasmamembraneitrelocalizestothelamellipodiaofastrocytomauponphosphorylationbyPRKCA.Localizestotheretractionfibers.Localizestotheplasmamembraneofoligodendrocytes(Bysimilarity).

        TISSUESPECIFICITY:Detectedonlyinmalignantmelanomacells.



        PTM:N-glycosylated(Bysimilarity).&O-glycosylated;containsglycosaminoglycanchondroitinsulfatewhicharerequiredforproperlocalizationandfunctioninstressfiberformation(Bysimilarity).InvolvedininteractionwithMMP16andITGA4.&PhosphorylationbyPRKCAregulatesitssubcellularlocationandfunctionincellmotility(Bysimilarity).


        SIMILARITY:SwissProt:Q6UVK1##Contains15CSPG(NG2)repeats.&Contains2lamininG-likedomains.

        MISCELLANEOUS:ValuableMarkerforseveralincompletelydifferentiatedprecursorcells.
        MolecularWeight~270-300kDa
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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