| Immunogen | KLH-conjugated,syntheticpeptide(REILSRRP(pS)YR)correspondingtoaminoacids125-135ofhumanCREB.Theimmunizingsequenceisidenticalinhumanandmouse.Clone10E9. |
| Clone | clone10E9 |
| Host | Mouse |
| Specificity | RecognizesphosphorylatedCREBatMr46kDa.Mayalsorecognizep30andp38proteins,whichlikelycorrespondtophosphorylatedCREMandATF-1respectively,sincethetwoproteinssharesignificanthomologywiththephosphorylatedCREBimmunizingpeptide |
| Isotype | IgG1κ |
| SpeciesReactivity | |
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | ThisgeneencodesatranscriptionfactorthatisamemberoftheleucinezipperfamilyofDNAbindingproteins.ThisproteinbindsasahomodimertothecAMP-responsiveelement,anoctamericpalindrome.Theproteinisphosphorylatedbyseveralproteinkinases,andinducestranscriptionofgenesinresponsetohormonalstimulationofthecAMPpathway.Alternatesplicingofthisgeneresultsintwotranscriptvariantsencodingdifferentisoforms. |
| GeneSymbol | |
| Modifications | |
| PurificationMethod | Thiophilicadsoptionandsizeexclusionchromatography |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P16220#ThisproteinbindsthecAMPresponseelement(CRE),asequencepresentinmanyviralandcellularpromoters.CREBstimulatestranscriptiononbindingtotheCRE. SIZE:341aminoacids;36688Da SUBUNIT:BindsDNAasadimer.InteractsPPRC1andwithHTLV-1Tax-1. SUBCELLULARLOCATION:Nucleus. PTM:Stimulatedbyphosphorylation.PhosphorylationofbothSer-133andSer-142intheSCNregulatestheactivityofCREBandparticipatesincircADIanrhythmgeneration.PhosphorylationofSer-133allowsCREBBPandEP300binding(Bysimilarity).PhosphorylateduponDNAdamage,probablybyATMorATR. SIMILARITY:SwissProt:P16220##BelongstothebZIPfamily.&Contains1bZIPdomain.&Contains1KID(kinase-inducIBLe)domain. |
| MolecularWeight | 46kDa |