| Immunogen | Fusionprotein,humanb-gal-episialin/MUC1repeatdomain |
| Clone | clone214D4 |
| Host | Mouse |
| Specificity | MUC1/episialin |
| Isotype | IgG1 |
| SpeciesReactivity | |
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Thisgeneisamemberofthemucinfamilyandencodesamembranebound,glycosylatedphosphoprotein.Theproteinisanchoredtotheapicalsurfaceofmanyepitheliabyatransmembranedomain,withthedegreeofglycosylationvaryingwithcelltype.Italsoincludesa20aavariablenumbertandemrepeat(VNTR)domain,withthenumberofrepeatsvaryingfrom20to120indifferentindividuals.Theproteinservesaprotectivefunctionbybindingtopathogensandalsofunctionsinacellsignalingcapacity.Overexpression,aberrantintracellularlocalization,andchangesinglycosylationofthisproteinhavebeenassociatedwithcarcinomas.Multiplealternativelysplicedtranscriptvariantsthatencodedifferentisoformsofthisgenehavebeenreported,butthefull-lengthnatureofonlysomehasbeendetermined. |
| GeneSymbol | - MUC1
- MUC1/ZD
- MUC-1/X
- PEMT
- MUC-1
- Episialin
- episialin
- MUC-1/SEC
- PUM
- EMA
- PEM
- H23AG
- MAM6
- CD227
|
| PurificationMethod | ProteinGChromatography |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P15941#Isoform7behavesasareceptorandbindsthesecretedisoform5.Thebindinginducesthephosphorylationoftheisoform7,alterscellularmorphologyandinitiatescellsignaling.CanbindtoGRB2adapterprotein. SIZE:1255aminoacids;122072Da SUBUNIT:Thecleavedform(onlytheisoform1)ofMUC1formsatightheterodimerwiththereleasedC-terminalpeptide(whichisfirstsecretedtobeextracellular). SUBCELLULARLOCATION:Cellmembrane;Single-passtypeImembraneprotein.&Isoform5:Secreted.&Isoform9:Secreted. TISSUESPECIFICITY:ExpressedonductalepithelialcellsandonactivatedT-cells.Aberrantlyglycosylatedformsexpressedinhumanepithelialtumors,suchasbreastorovariancancerandalsoinnon-epithelialtumorcells.Isoform7expressedonlyintumoralcells. PTM:Highlyglycosylated(N-andO-linkedcarbohydratesandsialicacid).Inthe20aminoacidtandemrepeatpositions5(Ser),6(Thr),14(Thr),15(Ser)and19(Thr)areO-glycosylated(GalNAc).TheaveragedensityofO-glycosylatedsiteswithinrepeatpeptidesvarieswithcelldifferentiationfromabout50%inlactation-associatedMUC1toover90%inavarietyofbreastcancercells.&Isoform1andisoform7undergoestransphosphorylationonserineandtyrosineresidues. SIMILARITY:SwissProt:P15941##Contains1SEAdomain. |
| MolecularWeight | nonegiven |