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    當前位置 : Millipore >>> Millipore/05-480 | Anti-Eck/EphA2 Antibody, clone D7/05-480/200 µg
    Millipore/05-480 | Anti-Eck/EphA2 Antibody, clone D7/05-480/200 µg
    • Millipore/05-480 | Anti-Eck/EphA2 Antibody, clone D7/05-480/200 µg

    Millipore/05-480 | Anti-Eck/EphA2 Antibody, clone D7/05-480/200 µg

    價格: ¥4812.00 市場價: 8020.00

    貨號: 05-480
    品牌: Millipore
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      • Description
        CatalogueNumber05-480
        BrandFamilyUpstate
        TradeName
        • Upstate
        DescriptionAnti-Eck/EphA2Antibody,cloneD7
        AlternateNames
        • EPHreceptorA2
        • Epithelialcellkinase
        • Tyrosine-proteinkinasereceptorECK
        • ephrinreceptorEphA2
        • epithelialcellreceptorproteintyrosinekinase
        • proteintyrosinekinase
        • receptorproteintyrosinekinaseregulatedbyp53andE2F-1
        BackgroundInformationEPHandEPH-relatedreceptorshavebeenimplicatedinmediatingdevelopmentalevents,particularlyinthenervoussystem.ReceptorsintheEPHsubfamilytypicallyhaveasinglekinasedomainandanextracellularregioncontainingaCys-richdomainand2fibronectintypeIIIrepeats.Theephrinreceptorsaredividedinto2groupsbasedonthesimilarityoftheirextracellulardomainsequencesandtheiraffinitiesforbindingephrin-Aandephrin-Bligands.
        Proteinkinasesareenzymesthattransferaphosphategroupfromaphosphatedonor,generallythegphosphateofATP,ontoanacceptoraminoacidinasubstrateprotein.Bythisbasicmechanism,proteinkinasesmediatemostofthesignaltransductionineukaryoticcells,regulatingcellularmetabolism,transcription,cellcycleprogression,cytoskeletalrearrangementandcellmovement,apoptosis,anddifferentiation.
        ProductInformation
        FormatPurified
        Control
        • A431celllysate,CHOcelllysate,foreskinfibroblasts,3T3/A31cells,L6cells.
          IncludedPositiveAntigenControl:Catalog#12-301,nonstimulatedA431lysate.Add2.5μLof2-mercaptoethanol/100μLoflysateandboilfor5minutestoreducethepreparation.Load20μgofreducedlysateperlaneforminigels.
        PresentationPurifiedmousemonoclonalIgG1inbuffercontaining0.2MTris-glycine,pH7.4,0.15MNaCl,with0.05%sodiumazideand30%glycerol.
        StorageandShippingInformation
        StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.

        HandlingRecommendations:
        Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.NOTE:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerol-containingsolutionstobecomefrozenduringstorage.
        Applications
        ApplicationThisAnti-Eck/EphA2Antibody,cloneD7isvalidatedforuseinIC,IP,EA,WBforthedetectionofEck/EphA2.
        KeyApplications
        • Immunocytochemistry
        • Immunoprecipitation
        • EnzymeAssays
        • WesternBlotting
        ApplicationNotesProteinKinaseAssay:
        Apreviouslotofthisantibodyhasbeenreportedtohavebeenusedinanimmunoprecipitationautophosphorylationassay,usingaMn-PIPESreactionbuffer(Romer,L.,1994).

        Immunoprecipitation:
        ApreviouslotofthisantibodyhasbeenreportedtoimmunoprecipitateEckfrom500μgofahumanbreastepithelialcelllinewhichhadbeenlysedinTBScontaining1%TritonX-100.Use1-4μgperreaction.

        Immunocytochemistry:
        ApreviouslotofthisantibodyhasbeenreportedtoimmunostainEckinhuman,mouseandratepithelialcellsfixedwith3.7%formaldehydesolutionandpermeabilizedwith0.5%TritonX-100inTBS.
        BIOLOGicalInformation
        ImmunogenNativeproteinisolatedbypurificationofphosphotyrosine-containingproteins.CloneD7.
        CloneD7
        ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
        HostMouse
        SpecificityThisantibodyrecognizesEpithelialCellKinase(Eck/EphA2),Mr140kDa
        IsotypeIgG1
        SpeciesReactivity
        • Human
        • Rat
        • Mouse
        • Bovine
        • Canine
        AntibodyTypeMonoclonalAntibody
        EntrezGeneNumber
        EntrezGeneSummaryThisgenebelongstotheephrinreceptorsubfamilyoftheprotein-tyrosinekinasefamily.EPHandEPH-relatedreceptorshavebeenimplicatedinmediatingdevelopmentalevents,particularlyinthenervoussystem.ReceptorsintheEPHsubfamilytypicallyhaveasinglekinasedomainandanextracellularregioncontainingaCys-richdomainand2fibronectintypeIIIrepeats.Theephrinreceptorsaredividedinto2groupsbasedonthesimilarityoftheirextracellulardomainsequencesandtheiraffinitiesforbindingephrin-Aandephrin-Bligands.Thisgeneencodesaproteinthatbindsephrin-Aligands.
        GeneSymbol
        • EPHA2
        • EphA2
        • ECK
        PurificationMethodProteinGPurified
        UniProtNumber
        UniProtSummaryFUNCTION:SwissProt:P29317#Receptorformembersoftheephrin-Afamily.Bindstoephrin-A1,-A3,-A4and-A5.
        SIZE:976aminoacids;108254Da
        SUBUNIT:InteractswithSLA(Bysimilarity).InteractswithINPPL1/SHIP2.
        SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein.
        TISSUESPECIFICITY:Expressedmosthighlyintissuesthatcontainahighproportionofepithelialcells,e.g.,skin,intestine,lung,andovary.
        SIMILARITY:SwissProt:P29317##Belongstotheproteinkinasesuperfamily.Tyrproteinkinasefamily.Ephrinreceptorsubfamily.&Contains2fibronectintype-IIIdomains.&Contains1proteinkinasedomain.&Contains1SAM(sterilealphamotif)domain.
        MolecularWeight140kDa
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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