| Immunogen | Nucleifrommyeloidleukemiabiopsycells.CloneAE-4. |
| Clone | AE-4 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Mouse |
| Specificity | ThisantibodyrecognizeshistoneH1,Mr32-33kDa.DoubletdetectedduetohyperphosphorylationofhistoneH1subpopulation.Cross-reactswithotherhistoneisoformsinthefollowingorderofsignalstrength:H3,H2B>H4>H2A,asdeterminedwithrecombinantprotein. |
| Isotype | IgG2a |
| SpeciesReactivity | |
| SpeciesReactivityNote | Humanandbovine.Predictedtostainnucleifromotheranimalandplantspecies. |
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Histonesarebasicnuclearproteinsresponsiblefornucleosomestructureofthechromosomalfiberineukaryotes.Twomoleculesofeachofthefourcorehistones(H2A,H2B,H3,andH4)formanoctamer,aroundwhichapproximately146bpofDNAiswrappedinrepeatingunits,callednucleosomes.Thelinkerhistone,H1,interactswithlinkerDNAbetweennucleosomesandfunctionsinthecompactionofchromatinintohigherorderstructures.ThisgeneisintronlessandencodesamemberofthehistoneH1family.TranscriptsfromthisgenelackpolyAtailsbutinsteadcontainapalindromicterminationelement.Thisgeneisfoundinthelargehistonegeneclusteronchromosome6. |
| GeneSymbol | - HIST1H1E
- H1F4
- H1.4
- dJ221C16.5
- MGC116819
|
| PurificationMethod | ProteinGPurified |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P10412#HistonesH1arenecessaryforthecondensationofnucleosomechainsintohigherorderstructures. SIZE:219aminoacids;21865Da SUBCELLULARLOCATION:Nucleus. SIMILARITY:SwissProt:P10412##BelongstothehistoneH1/H5family. MISCELLANEOUS:Thisvariantaccountsfor60%ofhistoneH1. |
| MolecularWeight | 32-33kDa(HistoneH1);Doublet(hyperphosphorylatedHistoneH1);17kDa(otherhistoneisoforms). |