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    當前位置 : Millipore >>> Millipore/05-412 | Anti-GSK3 Antibody, clone 4G-1E/05-412/100 µg
    Millipore/05-412 | Anti-GSK3 Antibody, clone 4G-1E/05-412/100 µg
    • Millipore/05-412 | Anti-GSK3 Antibody, clone 4G-1E/05-412/100 µg

    Millipore/05-412 | Anti-GSK3 Antibody, clone 4G-1E/05-412/100 µg

    價格: ¥4308.00 市場價: 7180.00

    貨號: 05-412
    品牌: Millipore
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      • Description
        CatalogueNumber05-412
        BrandFamilyUpstate
        TradeName
        • Upstate
        DescriptionAnti-GSK3Antibody,clone4G-1E
        AlternateNames
        • GSK-3alpha
        • glycogensynthasekinase3alpha
        BackgroundInformationGlycogensynthasekinase-3(GSK-3),amultifunctionalserine/threoninekinase,isakeyregulatorofnumeroussignalingpathways.TwoisoformsofGSK-3arereportedinmammals:a51kDaGSK-3aanda47kDaGSK-3b.Thesetwoisoformsexhibitabout98%homologyintheirkinasedomains,butshareonlyabout36%identityinthelast76C-terminalaminoacidresidues.GSK-3acontainsaglycine-richextensionatitsN-terminus.Aminor(~15%oftotal)splicevariantofGSK-3b,GSK-3b2,hasalsobeenidentified,whichcontainsa13-residueinsertwithinthekinasedomain.Itexhibitsreducedkinaseactivitytowardstauproteincomparedwith‘unspliced’GSK-3b.GSK-3b2islocalizedprimarilytoneuronalcellbodies,unlikeunsplicedGSK-3bthatisalsofoundinneuronalprocesses.GSK-3bplaysakeyinhibitoryroleintheWntsignalingpathway.Wntgenesencodealargefamilyofsecreted,cysteine-richproteinsthatareimportantindevelopmentandinmaintenanceofadulttissues.AbnormalitiesinWntsignalingarereportedtopromotebothhumandgenerativediseasesandcancer.
        ProductInformation
        FormatPurified
        Control
        • Jurkatecelllysate,293Tcellextract,rabbitspleenfibroblasts

          IncludedPositiveAntigenControl:
          Catalog#12-303,Jurkatlysate.Add2.5μLof2-mercaptoethanolper100μLoflysateandboilfor5minutestoreducethepreparation.Use20μgperlaneforminigels.
        PresentationProteinGpurifiedmouseIgG1instoragebuffercontaining0.02Mphosphatebuffer,pH7.6,0.25MNaCl,and0.1%sodiumazide.
        StorageandShippingInformation
        StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.
        HandlingRecommendations:Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
        Applications
        ApplicationAnti-GSK3Antibody,clone4G-1EdetectslevelofGSK3&hasbeenpublished&validatedforuseinIC&WB.
        KeyApplications
        • Immunocytochemistry
        • WesternBlotting
        ApplicationsNotRecommended
        • Immunoprecipitation
        ApplicationNotesImmunoprecipitation:
        Notrecommended.

        Immunocytochemistry:
        10μg/mLofapreviouslotshowedpositiveimmunostainingforGSK3inL6cellsfixedwithacetone.
        BIOLOGicalInformation
        Immunogen18residuesyntheticpeptide(C-KQLLHGEPNVSYICSRY)correspondingtoaminoacids203-219derivedfromaregionofthecatalyticdomain(betweensuBDomainVIIandVIII)oftheDrosophilaGSK3/shaggyenzyme,coupledtoovalbumin.Clone4G-1E.
        Clone4G-1E
        ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
        HostMouse
        SpecificityGSK3,αandβisoforms
        IsotypeIgG1
        SpeciesReactivity
        • Vertebrates
        • Mouse
        • Rat
        • Human
        • Dictyostelium
        • Yeast(S.pombe)
        SpeciesReactivityNoteMouse,rat,S.pombe,Dictyosteliumandhuman.Becauseoftheextremelyhighconservationofthisimmunogenamongtheknownenzymesofmanydifferentspecies,thisantibodyispredictedtocross-reactwithnearlyallspecieshomologues.
        AntibodyTypeMonoclonalAntibody
        EntrezGeneNumber
        EntrezGeneSummaryGlycogensynthasekinase3-alpha(GSK3A;EC{2.7.1.37})isamultifunctionalproteinserinekinase,homologoustoDrosophila"shaggy"(zeste-white3)andimplicatedinthecontrolofseveralregulatoryproteinsincludingglycogensynthase(seeGYS1,{138570})andtranscriptionfactors(e.g.,JUN,{165160}).ItalsoplaysaroleintheWNT({164820})andPI3K(seePIK3CG,{601232})signalingpathways(seereviewby{1:Alietal.(2001)}).[suppliedbyOMIM]
        GeneSymbol
        • GSK3A
        • DKFZp686D0638
        PurificationMethodProteinGPurified
        UniProtNumber
        UniProtSummaryFUNCTION:SwissProt:P49840#Implicatedinthehormonalcontrolofseveralregulatoryproteinsincludingglycogensynthase,MYBandthetranscriptionfactorJUN(Bysimilarity).
        SIZE:483aminoacids;50981Da
        SUBUNIT:Monomer(Bysimilarity).
        SIMILARITY:SwissProt:P49840##Belongstotheproteinkinasesuperfamily.CMGCSer/Thrproteinkinasefamily.GSK-3subfamily.&Contains1protein
        MolecularWeight51kDa(α)or46kDa(β)
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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