| Immunogen | GSTfusionproteincorrespondingtoaminoacids508-616ofhumanSirt1. |
| Clone | 3H10.2 |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Mouse |
| Specificity | HumanSirt1.DoesnotcrossreactwithlysatesfrommouseNIH/3T3cellsorratPC12cells. |
| Isotype | IgG1 |
| SpeciesReactivity | |
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Thisgeneencodesamemberofthesirtuinfamilyofproteins,homologstotheyeastSir2protein.Membersofthesirtuinfamilyarecharacterizedbyasirtuincoredomainandgroupedintofourclasses.Thefunctionsofhumansirtuinshavenotyetbeendetermined;however,yeastsirtuinproteinsareknowntoregulateepigeneticgenesilencingandsuppressrecombinationofrDNA.Studiessuggestthatthehumansirtuinsmayfunctionasintracellularregulatoryproteinswithmono-ADP-ribosyltransferaseactivity.TheproteinencodedbythisgeneisincludedinclassIofthesirtuinfamily. |
| GeneSymbol | - SIRT1
- SIR2L1
- hSIR2
- OTTHUMP00000060745
- hSIRT1
- SIR2alpha
|
| PurificationMethod | ProteinGPurified |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:Q96EB6#NAD-dependentdeacetylase,whichregulatesprocessessuchasapoptosisandmuscledifferentiationbydeacetylatingkeyproteins.Deacetylates"Lys-382"ofp53/TP53andimpairsitsABIlitytoinduceproapoptoticprogramandmodulatecellsenescence.DeacetylatesTAF1BandtherebyrepressesrDNAtranscriptionbytheRNApolymeraseI.InvolvedinHES1-andHEY2-mediatedtranscriptionalrepression.InhibitsskeletalmuscledifferentiationbydeacetylatingPCAFandMYOD1.MayserveasasensorofthecytosolicratioofNAD(+)/NADH,whichisessentialinskeletalmusclecelldifferentiation.Despitesomeabilitytodeacetylatehistonesinvitro,suchactivityiseitherweakorinexistentinvivo.IncaseofHIV-1infection,interactswithanddeacetylatestheviralTatprotein. COFACTOR:Binds1zincionpersubunit(Bysimilarity). SIZE:747aminoacids;81681Da SUBUNIT:InteractswithTAF1B.FoundinacomplexwithPCAFandMYOD1(Bysimilarity).InteractswithMLLT7/FOXO4,HES1,HEY2,p53/TP53andPML. SUBCELLULARLOCATION:Nucleus.Note=RecruitedtothenuclearbodiesviaitsinteractionwithPML.TISSUESPECIFICITY:Widelyexpressed. SIMILARITY:SwissProt:Q96EB6##Belongstothesirtuinfamily.&Contains1deacetylasesirtuin-typedomain. MISCELLANEOUS:Redwine,whichcontainsresveratrol,mayparticipateinactivationofsirtuinproteins,andmaythereforeparticipateinanextendedlifespanasithasbeenobservedinyeast. |
| MolecularWeight | 120kDa |