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    當前位置 : Millipore >>> Millipore/05-1243 | Anti-Sirt1 Antibody, clone 3H10.2/05-1243/100 µg
    Millipore/05-1243 | Anti-Sirt1 Antibody, clone 3H10.2/05-1243/100 µg
    • Millipore/05-1243 | Anti-Sirt1 Antibody, clone 3H10.2/05-1243/100 µg

    Millipore/05-1243 | Anti-Sirt1 Antibody, clone 3H10.2/05-1243/100 µg

    價格: ¥5052.00 市場價: 8420.00

    貨號: 05-1243
    品牌: Millipore
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      • Description
        CatalogueNumber05-1243
        Replaces05-707
        BrandFamilyUpstate
        TradeName
        • Upstate
        DescriptionAnti-Sirt1Antibody,clone3H10.2
        BackgroundInformationTheSir2proteininyeastisknowntofunctionintranscriptionalsilencingprocessesthroughthedeacetylationofhistonesH3andH4.ThemorerecentlydescribedhumanhomologueofSir2,knownasSIRT1,hasbeenfoundtoassociatewiththetumorsuppressorproteinp53.TherearenowfivedescribedhumanhomologsofyeastSir2,whicharenamedSIRT1-5.
        SIRT1bindsanddeacetylatesp53withspecificityforitsC-terminalLys382residueinresponsetotheupregulationofpromyelocyticleukemiaprotein(PML)nuclearbodiesoroncogenicRas.Thedeacetylationofp53SIRT1hasbeenshowntonegativelyregulatep53-mediatedtranscription,preventingcellularsenescenceandapoptosisinducedbyDNAdamageandstress.SIRT1isaHDACwhichisimportantinestablishingrepressivechromatinstructuresandplaysaroleinincreasingLifespan.
        ProductInformation
        FormatPurified
        PresentationPurifiedinPBSwith0.05%SodiumAzide
        StorageandShippingInformation
        StorageConditionsMaintainat2-8°Cinundilutedaliquotsforupto6monthsafterdateofreceipt.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
        Applications
        ApplicationAnti-Sirt1Antibody,clone3H10.2isaMouseMonoclonalAntibodyfordetectionofSirt1alsoknownasSirtuin1,SilentmatingtypeInformationRegulation-2&hasbeentestedinIP&WB.
        KeyApplications
        • Immunoprecipitation
        • WesternBlotting
        BIOLOGicalInformation
        ImmunogenGSTfusionproteincorrespondingtoaminoacids508-616ofhumanSirt1.
        Clone3H10.2
        ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
        HostMouse
        SpecificityHumanSirt1.DoesnotcrossreactwithlysatesfrommouseNIH/3T3cellsorratPC12cells.
        IsotypeIgG1
        SpeciesReactivity
        • Human
        AntibodyTypeMonoclonalAntibody
        EntrezGeneNumber
        EntrezGeneSummaryThisgeneencodesamemberofthesirtuinfamilyofproteins,homologstotheyeastSir2protein.Membersofthesirtuinfamilyarecharacterizedbyasirtuincoredomainandgroupedintofourclasses.Thefunctionsofhumansirtuinshavenotyetbeendetermined;however,yeastsirtuinproteinsareknowntoregulateepigeneticgenesilencingandsuppressrecombinationofrDNA.Studiessuggestthatthehumansirtuinsmayfunctionasintracellularregulatoryproteinswithmono-ADP-ribosyltransferaseactivity.TheproteinencodedbythisgeneisincludedinclassIofthesirtuinfamily.
        GeneSymbol
        • SIRT1
        • SIR2L1
        • hSIR2
        • OTTHUMP00000060745
        • hSIRT1
        • SIR2alpha
        PurificationMethodProteinGPurified
        UniProtNumber
        UniProtSummaryFUNCTION:SwissProt:Q96EB6#NAD-dependentdeacetylase,whichregulatesprocessessuchasapoptosisandmuscledifferentiationbydeacetylatingkeyproteins.Deacetylates"Lys-382"ofp53/TP53andimpairsitsABIlitytoinduceproapoptoticprogramandmodulatecellsenescence.DeacetylatesTAF1BandtherebyrepressesrDNAtranscriptionbytheRNApolymeraseI.InvolvedinHES1-andHEY2-mediatedtranscriptionalrepression.InhibitsskeletalmuscledifferentiationbydeacetylatingPCAFandMYOD1.MayserveasasensorofthecytosolicratioofNAD(+)/NADH,whichisessentialinskeletalmusclecelldifferentiation.Despitesomeabilitytodeacetylatehistonesinvitro,suchactivityiseitherweakorinexistentinvivo.IncaseofHIV-1infection,interactswithanddeacetylatestheviralTatprotein.
        COFACTOR:Binds1zincionpersubunit(Bysimilarity).
        SIZE:747aminoacids;81681Da
        SUBUNIT:InteractswithTAF1B.FoundinacomplexwithPCAFandMYOD1(Bysimilarity).InteractswithMLLT7/FOXO4,HES1,HEY2,p53/TP53andPML.
        SUBCELLULARLOCATION:Nucleus.Note=RecruitedtothenuclearbodiesviaitsinteractionwithPML.TISSUESPECIFICITY:Widelyexpressed.
        SIMILARITY:SwissProt:Q96EB6##Belongstothesirtuinfamily.&Contains1deacetylasesirtuin-typedomain.
        MISCELLANEOUS:Redwine,whichcontainsresveratrol,mayparticipateinactivationofsirtuinproteins,andmaythereforeparticipateinanextendedlifespanasithasbeenobservedinyeast.
        MolecularWeight120kDa
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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