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    當前位置 ： Millipore >>> Millipore/04-349 | Anti-MAP Kinase 2/Erk2 Antibody, clone E460, rabbit monoclonal/04-349/100 µL
    Millipore/04-349 | Anti-MAP Kinase 2/Erk2 Antibody, clone E460, rabbit monoclonal/04-349/100 µL
    • Millipore/04-349 | Anti-MAP Kinase 2/Erk2 Antibody, clone E460, rabbit monoclonal/04-349/100 µL

    Millipore/04-349 | Anti-MAP Kinase 2/Erk2 Antibody, clone E460, rabbit monoclonal/04-349/100 µL

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    貨號: 04-349
    品牌: Millipore
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      • Description
        CatalogueNumber04-349
        DescriptionAnti-MAPKinase2/Erk2Antibody,cloneE460,rabbitmonoclonal
        AlternateNames
        • mitogen-activatedproteinkinase1
        • Extracellularsignal-regulatedkinase2
        • Mitogen-activatedproteinkinase2
        • MAPkinase1
        • MAPkinase2
        • MAPkinaseisoformp42
        • proteintyrosinekinaseERK2
        BackgroundInformationExtracellularsignalregulatedkinase2(ERK2)alsoknownasmaturationormitogenactivatedproteinkinase(MAPK)isinvolvedineukaryoticsignaltransduction(1).Activatedbyadualphosphorylationonthreonineandtyrosineresiduebythedual-specificitykinaseMKK1/2,ERK2p42translocatetothenucleusafterstimulation(2).Oncephosphorylated,ERK2canformdimerswithphosphorylatedandunphosphorylatedERK2partners.ItisalsoabletophosphorylatemultiplesubstratessuchasEGFR,c-PLA2,Elk1/TCFandc-Myc(3-5).
        ProductInformation
        FormatUnpurified
        Control
        • A431celllysate
        PresentationRabbitMonoclonalinbuffercontainingglycerol,BSA,andsodiumazide.
        StorageandShippingInformation
        StorageConditionsStablefor1yearat-20oCfromdateofreceipt.
        HandlingRecommendations:Uponfirstthaw,andpriortoremovingcap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20oC.Avoidfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
        Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.Note:Variabilityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.
        Applications
        ApplicationPleasenotethatthisproductwillnotbeavailableforsaleafterMarch15,2015.Pleaseselectoneoftheotherantibodiesagainstthistarget.
        KeyApplications
        • WesternBlotting
        • Immunocytochemistry
        • Immunoprecipitation
        • FlowCytometry
        • Immunohistochemistry(Paraffin)
        ApplicationNotesImmunohistochemistryAnalysis:A1:250-500dilutionfromarepresentativelotwasusedinhumanbreastcarcinomatissue.
        ImmunocytochemistryAnalysis:A1:100-250dilutionfromarepresentativelotwasusedinIC.
        ImmunoprecipitationAnalysis:A1:80dilutionfromarepresentativelotwasusedinIP.
        FlowCytometryAnalysis:A1:50dilutionfromarepresentativelotwasusedinFC.
        BIOLOGicalInformation
        ImmunogenSyntheticpeptidecorrespondingtotheC-terminusofhumanMAPKinase2/Erk2.
        EpitopeC-terminus
        CloneE460
        HostRabbit
        IsotypeIgG
        SpeciesReactivity
        • Human
        • Mouse
        • Rat
        AntibodyTypeMonoclonalAntibody
        EntrezGeneNumber
        GeneSymbol
        • ERK2
        • PRKM1
        • ERK
        • MAPK2
        • p41mapk
        • ERK-2
        • ERT1
        • PRKM2
        • MAPK2
        • p42-MAPK
        • EC2.7.11.24
        • OTTHUMP00000028754
        • OTTHUMP00000174492
        • P42MAPK
        • p38
        • p40
        • p41
        • MAPK1
        • EC2.7.11
        PurificationMethodUnpurified
        UniProtNumber
        UniProtSummaryFUNCTION:Serine/threoninekinasewhichactsasanessentialcomponentoftheMAPkinasesignaltransductionpathway.MAPK1/ERK2andMAPK3/ERK1arethe2MAPKswhichplayanimportantroleintheMAPK/ERKcascade.TheyparticipatealsoinasignalingcascadeinitiatedbyactivatedKITandKITLG/SCF.Dependingonthecellularcontext,theMAPK/ERKcascademediatesdiversebiologicalfunctionssuchascellgrowth,adhesion,survivalanddifferentiationthroughtheregulationoftranscription,translation,cytoskeletalrearrangements.ThetheMAPK/ERKcascadeplaysalsoaroleininitiationandregulationofmeiosis,mitosis,andpostmitoticfunctionsindifferentiatedcellsbyphosphorylatinganumberoftranscriptionfactors.About160substrateshavealreadybeendiscoveredforERKs.Manyofthesesubstratesarelocalizedinthenucleus,andseemtoparticipateintheregulationoftranscriptionuponstimulation.However,othersubstratesarefoundinthecytosolaswellasinothercellularorganelles,andthoseareresponsIBLeforprocessessuchastranslation,mitosisandapoptosis.Moreover,theMAPK/ERKcascadeisalsoinvolvedintheregulationoftheendosomaldynamics,includinglysosomeprocessingandendosomecyclingthroughtheperinuclearrecyclingcompartment(PNRC);aswellasinthefragmentationoftheGolgiapparatusduringmitosis.Thesubstratesincludetranscriptionfactors(suchasATF2,BCL6,ELK1,ERF,FOS,HSF4orSPZ1),cytoskeletalelements(suchasCANX,CTTN,GJA1,MAP2,MAPT,PXN,SORBS3orSTMN1),regulatorsofapoptosis(suchasBAD,BTG2,CASP9,DAPK1,IER3,MCL1orPPARG),regulatorsoftranslation(suchasEIF4EBP1)andavarietyofothersignaling-relatedmolecules(likeARHGEF2,FRS2orGRB10).Proteinkinases(suchasRAF1,RPS6KA1/RSK1,RPS6KA3/RSK2,RPS6KA2/RSK3,RPS6KA6/RSK4,SYK,MKNK1/MNK1,MKNK2/MNK2,RPS6KA5/MSK1,RPS6KA4/MSK2,MAPKAPK3orMAPKAPK5)andphosphatases(suchasDUSP1,DUSP4,DUSP6orDUSP16)areothersubstrateswhichenablethepropagationtheMAPK/ERKsignaltoadditionnalcytosolicandnucleartargets,therebyextendingthespecificityofthecascade.
        Actsasatranscriptionalrepressor.Bindstoa[GC]AAA[GC]consensussequence.Represstheexpressionofinterferongamma-inducedgenes.SeemstobindtothepromoterofCCL5,DMP1,IFIH1,IFITM1,IRF7,IRF9,LAMP3,OAS1,OAS2,OAS3andSTAT1.Transcriptionalactivityisindependentofkinaseactivity.
        CATALYTICACTIVITY:ATP+aprotein=ADP+aphosphoprotein.
        COFACTOR:MagnesiumBysimilarity.
        ENZYMEREGULATION:PhosphorylatedbyMAP2K1/MEK1andMAP2K2/MEK2onThr-185andTyr-187inresponsetoexternalstimulilikeinsulinorNGF.Bothphosphorylationsarerequiredforactivity.Thisphosphorylationcausesdramaticconformationalchanges,whichenablefullactivationandinteractionofMAPK1/ERK2withitssubstrates.PhosphorylationonSer-29bySGK1resultsinitsactivationbyenhancingitsinteractionwithMAP2K1/MEK1andMAP2K2/MEK2.DephospphorylatedandinactivatedbyDUSP3,DUSP6andDUSP9.Inactivatedbypyrimidylpyrroleinhibitors.
        SUBUNITSTRUCTURE:Bindsbothupstreamactivatorsanddownstreamsubstratesinmultimolecularcomplexes.BindstoHIV-1NefthroughitsSH3domain.Thisinteractioninhibitsitstyrosine-kinaseactivity.InteractswithADAM15,ARHGEF2,ARRB2,DAPK1(viadeathdomain),HSF4,IER3,IPO7,DUSP6,NISCH,SGK1,andisoform1ofNEK2.Interacts(phosphorylatedform)withCAV2("Tyr-19"-phosphorylatedform);theinteraction,promotedbyinsulin,leadstonuclearlocationandMAPK1activation.InteractswithMORG1,PEA15andMKNK2Bysimilarity.MKNK2isoform1bindingpreventsfromdephosphorylationandinactivationBysimilarity.
        SUBCELLULARLOCATION:Nucleus.Cytoplasm?Cytoskeleton?centrosome.Cytoplasm.Note:PEA15-bindingandphosphorylatedDAPK1promoteitscytoplasmicretention.PhosphorylationatSer-246andSer-248aswellasautophosphorylationatThr-190promotenuclearlocalization.
        DOMAIN:TheTXYmotifcontainsthethreonineandtyrosineresidueswhosephosphorylationactivatestheMAPkinases.
        PTM:PhosphorylateduponKITandFLT3signalingBysimilarity.DuallyphosphorylatedonThr-185andTyr-187,whichactivatestheenzyme.UndergoesregulatoryphosphorylationonadditionalresiduessuchasSer-246andSer-248inthekinaseinsertdomain(KID)Thesephosphorylations,whichareprobablymediatedbymorethanonekinase,areimportantforbindingofMAPK1/ERK2toimportin-7(IPO7)anditsnucleartranslocation.Inaddition,autophosphorylationofThr-190wasshowntoaffectthesubcellularlocalizationofMAPK1/ERK2aswell.Ligand-activatedALKinducestyrosinephosphorylation.DephosphorylatedbyPTPRJatTyr-187.PhosphorylationonSer-29bySGK1resultsinitsactivationbyenhancingitsinteractionwithMAP2K1/MEK1andMAP2K2/MEK2.DUSP3andDUSP6dephosphorylatespecificallyMAPK1/ERK2andMAPK3/ERK1whereasDUSP9dephosphorylatesabroaderrangeofMAPKs.
        SEQUENCESIMILARITIES:Belongstotheproteinkinasesuperfamily.CMGCSer/Thrproteinkinasefamily.MAPkinasesubfamily.
        Contains1proteinkinasedomain.
        SEQUENCECAUTION:ThesequenceCAA77753.1differsfromthatshown.Reason:Erroneousinitiation.TranslationN-terminallyextended.
        MolecularWeight~42kDaobserved
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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