| Immunogen | SyntheticpeptidecorrespondingtoresiduesontheC-terminusofhumanSIRT2. | 
| Epitope | C-terminus | 
| Clone | EP1668Y | 
| Host | Rabbit | 
| Specificity | ThisantibodyrecognizesSIRT2atandaroundtheC-terminus,allthreeisoforms. | 
| Isotype | IgG | 
| SpeciesReactivity |  | 
| AntibodyType | MonoclonalAntibody | 
| EntrezGeneNumber |  | 
| EntrezGeneSummary | Thisgeneencodesamemberofthesirtuinfamilyofproteins,homologstotheyeastSir2protein.Membersofthesirtuinfamilyarecharacterizedbyasirtuincoredomainandgroupedintofourclasses.Thefunctionsofhumansirtuinshavenotyetbeendetermined;however,yeastsirtuinproteinsareknowntoregulateepigeneticgenesilencingandsuppressrecombinationofrDNA.Studiessuggestthatthehumansirtuinsmayfunctionasintracellularregulatoryproteinswithmono-ADP-ribosyltransferaseactivity.TheproteinencodedbythisgeneisincludedinclassIofthesirtuinfamily.Twotranscriptvariantsresultfromalternativesplicingofthisgene. | 
| GeneSymbol | - EC3.5.1.-
 - SIR2
 - SIR2-like
 - SIR2L
 - SIR2L2
 
  | 
| PurificationMethod | Unpurified | 
| UniProtNumber |  | 
| UniProtSummary | FUNCTION:NAD-dependentdeacetylase,whichdeacetylatesthe"Lys-40"ofalpha-tubulin.Involvedinthecontrolofmitoticexitinthecellcycle,probablyviaitsroleintheregulationofCytoskeleton.Despitesomeabilitytodeacetylatehistonesinvitro,itisunlikelyinvivo. CATALYTICACTIVITY:NAD++anacetylprotein=nicotinamide+O-acetyl-ADP-ribose+aprotein. COFACTOR:Binds1zincionpersubunit. ENZYMEREGULATION:InhibitedbySirtinol,A3andM15smallmolecules.Inhibitedbynicotinamide. SUBUNITSTRUCTURE:InteractswithHDAC6,suggestingthattheseproteinsbelongtoalargecomplexthatdeacetylatethecytoskeleton. SUBCELLULARLOCATION:Cytoplasm?cytoskeleton.Note:Colocalizeswithmicrotubules. TISSUESPECIFICITY:Widelyexpressed.Highlyexpressedinheart,brainandskeletalmuscle,whileitisweaklyexpressedinplacentaandlung.Down-regulatedinmanygliomassuggestingthatitmayactasatumorsuppressorgeneinhumangliomaspossiblythroughtheregulationofmicrotubulenetwork. DEVELOPMENTALSTAGE:Peaksduringmitosis.Aftermitosis,itisprobablydegradedbythe26Sproteasome. PTM:PhosphorylatedattheG2/Mtransitionofthecellcycle. SEQUENCESIMILARITIES:Belongstothesirtuinfamily. Contains1deacetylasesirtuin-typedomain. SEQUENCECAUTION:ThesequenceAAF67015.1differsfromthatshown.Reason:Frameshiftatseveralpositions. | 
| MolecularWeight | ~36-43kDa(detectsall3isoforms) |