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    當前位置 ： NEB >>> NEB/Monarch? PCR & DNA Cleanup Kit (5 μg)/T1030S/50 preps
    NEB/Monarch? PCR & DNA Cleanup Kit (5 μg)/T1030S/50 preps
    • NEB/Monarch? PCR & DNA Cleanup Kit (5 μg)/T1030S/50 preps

    NEB/Monarch? PCR & DNA Cleanup Kit (5 μg)/T1030S/50 preps

    價格: ￥1188.00 市場價: 1980.00
    打印產品信息
    貨號: T1030S-50preps
    品牌: NEB
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      • Description:

        ?
        TheMonarchPCR&DNACleanupKitrapidlyandreliablypurifiesupto5μgofconcentrated,high-qualityDNAfromPCRandotherenzymaticreactions.Thekitutilizesabind/wash/eluteworkflowwithminimalincubationandspintimes.Thecolumnsensurezerobufferretentionandnocarryoverofcontaminants,enablingelutionofsampleinvolumesaslowas6μl.Thebuffersprovidedhavebeenoptimized,anddonotrequiremonitoringofpH.ElutedDNAisreadyforuseinrestrictiondigests,DNAsequencing,ligationandotherenzymaticmanipulations.DesignedwithsustainABIlityinmind,MonarchkitsusesignificantlylessplasticandresponsIBLy-sourced,recyclablepackaging.TheprotocolcanalsobemodifiedtoenablethepurificationofsmallerDNAfragments,includingoligonucleotides.

        Viewourvideosonprotocols,tips,andrecyclingMonarch.

        ?
        APPLICATIONS
        PCRcleanupDNAfromPCRreactionscanbepurifiedafteramplificationtoremovepolymerases,primers,detergents,dNTPs,etc.
        EnzymaticreactioncleanupModifyingenzymessuchasligases,kinases,nucleases,phosphatasesareefficientlyremovedallowingefficientdesaltingandconcentrationoftheDNAsample.
        CDNAcleanupDNA/RNAcomplexescanbepurifiedpost-reversetranscription/amplificationtoenableremovaloftheRTandpolymeraseaswellasnucleotides.
        LabelingcleanupUnincorporatedrADIolabeledorfluorescentlylabelednucleotidescanberemovedfromtheDNAsubstrate
        PlasmidcleanupPlasmidprepsfromunknownsourcesmaycontaininhibitorsandunwantedcontaminants.Purificationandconcentrationcanbeeasilyachievedusingthiskit.
        OligonucleotidecleanupssDNAoligonucleotides(≥18nt)anddsDNAfragments(≥?15bp)canbepurifiedusingtheOligonucleotideCleanupProtocol.


        MonarchDNACleanupColumnDesign
        Monarch DNA Cleanup Column Design

        Monarchcolumnsaredesignedforperformance
        Monarch columns are designed for performance. Monarch columns are designed without a frit, which eliminates buffer retention and the risk of carryover contamination, providing fast, worry-free DNA purification.
        Monarchcolumnsaredesignedwithoutafrit,whicheliminatesbufferretentionandtheriskofcarryovercontamination,providingfast,worry-freeDNApurification.

        ?Advantages:
        • Eluteinaslittleas6μl
        • Preventbufferretentionandsaltcarry-overwithoptimizedcolumndesign
        • Savetimewithfast,user-friendlyprotocols
        • NoneedtomonitorpH
        • Buffersandcolumnsavailableseparately
        • Significantlylessplasticusedwhencomparedwithotherkits?
        • Responsibly-sourcedandrecyclablepackaging?

        Specifications

        DNASampleType:DNAfromPCRandotherenzymaticreactions(e.g.,restrictiondigests,kinasereactions,ligations).*
        BindingCapacity:upto5μg
        DNASizeRange:~50bpto25kb**
        TypicalRecovery:DNA(50bpto10kb):70–90%/?DNA(11–23kb):50–70%
        ElutionVolume:≥6μl
        Purity:A260/280>1.8andA260/230>1.8
        ProtocolTime:5minutesofspinandincubationtime
        CompatibleDownstream
        Applications:        		ligation,restrictiondigestion,labelingandotherenzymatic
        manipulations,libraryconstructionandDNAsequencing.

        *ssDNAordsDNAoligonucleotidesfromenzymaticreactionscanalsobepurifiedusingtheOligonucleotideCleanupProtocol.
        **DNA≥15bpto25kb(dsDNA)andDNA≥18ntto10kb(ssDNA)canalsobepurifiedusingtheOligonucleotideCleanupProtocol.
        ?

        MonarchPCR&DNACleanupKit(5μg)Protocol?
        Monarch PCR & DNA Cleanup Kit (5 μg) Protocol

        MonarchPCR&DNACleanupKit(5μg)performsequivalentlytotheleadingsupplier
        Monarch PCR & DNA Cleanup Kit (5 μg) performs equivalently to the leading supplier. Preps were performed according to recommended protocols. 1 μg of a 3 kb DNA fragment was incubated with 1 μM primers and OneTaq? Quick-Load? 2X Master Mix (NEB #M0486). DNA was eluted in 20 μl (NEB) and 40 μl (Qiagen) Elution Buffer. Half of the total elution volume was digested with 5 units of DraIII-HF? (NEB #R3510). The digest and the unused portion of the elution were resolved on a 1% w/v agarose gel along with a representative sample of the starting material.
        Prepswereperformedaccordingtorecommendedprotocols.1μgofa3kbDNAfragmentwasincubatedwith1μMprimersandOneTaq?Quick-Load?2XMasterMix(NEB#M0486).DNAwaselutedin20μl(NEB)and40μl(Qiagen)ElutionBuffer.Halfofthetotalelutionvolumewasdigestedwith5unitsofDraIII-HF?(NEB#R3510).Thedigestandtheunusedportionoftheelutionwereresolvedona1%w/vagarosegelalongwitharepresentativesampleofthestartingmaterial.?
        MonarchPCR&DNACleanupKit(5μg)removeslowmolecularweightprimersfromdsDNAsamples
        Monarch PCR & DNA Cleanup Kit (5 μg) removes low molecular weight primers from dsDNA samples. Three independent amplicons (267 bp, 520 bp, 1003 bp) were spiked with two oligonucleotides (16-mer, 24-mer) to a final concentration of 1 μM. Half of each mix was purified with the Monarch PCR & DNA Cleanup Kit (5 μg) following the included protocol. Equivalent fractions of the original mixture and the eluted material were resolved on a 20% TBE acrylamide gel at 100V for one hour and stained with SYBR Green II.
        Threeindependentamplicons(267bp,520bp,1003bp)werespikedwithtwooligonucleotides(16-mer,24-mer)toafinalconcentrationof1μM.HalfofeachmixwaspurifiedwiththeMonarchPCR&DNACleanupKit(5μg)followingtheincludedprotocol.Equivalentfractionsoftheoriginalmixtureandtheelutedmaterialwereresolvedona20%TBEacrylamidegelat100VforonehourandstainedwithSYBRGreenII.

        LearnMoreAboutMonarch
        • NEBMonarch.com
        • Optimizeyourresultswithouruniquecolumndesign
        • EnhanceyourDNApurificationexperience
        • FeelgoodaboutchoosingMonarch
        • ChooseMonarchkitsforpurevalue

        KitComponents

        Thefollowingreagentsaresuppliedwiththisproduct:

        Storeat(°C)Concentration
        Monarch®DNACleanupBindingBuffer251X
        Monarch®DNAWashBuffer255X
        Monarch®DNAElutionBuffer251X
        Monarch®DNACleanupColumns(5μg)25

        Notes:

        Thekitshouldbestoredatroomtemperature.Alwayskeepbufferbottlestightlyclosedandkeepcolumnssealedintheenclosedzip-lockbag.Forinformationregardingthecompositionofbuffers,pleaseconsulttheSafetyDataSheets.Properlaboratorysafetypracticesshouldbeemployed,includingtheuseoflabcoats,glovesandeyeprotection.
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