Description
Qdot®655streptavidinconjugatecomprisesabiotin-bindingprotein(streptavidin)covalentlyattachedtoafluorescentlabel(Qdot®nanocrystal).Streptavidinhasaveryhighbindingaffinityforbiotin,andaconjugateofstreptavidiniscommonlyusedtogetherwithaconjugateofbiotinforspecificdetectionofavarietyofproteins,proteinmotifs,nucleicacids,andothermolecules(forexample,abiotinylatedprimaryantibodyboundtoaproteintargetcanbedetectedwithafluorescentlylabeledstreptavidin).Strategiessimilartothisareusedinmanydetectionprotocolsincludingwesternblots,flowcytometry,imagingandmicroscopy,andmicroplateassays,andarealsousedinpurificationworkflowstoachievetargetedfractionation.Qdot®nanocrystalconjugatesareprovidedas1µMsolutions.
ImportantFeaturesofQdot®StreptavidinConjugates:•
StreptavidinQdot®655conjugatehasemissionmaximaof~655nm•
Approximately5to10streptavidinsperQdot®nanocrystal•
Extremelyphotostableandbrightfluorescence•
Efficientlyexcitedwithsingle-lineexcitationsources•
Narrowemission,largeStokesshift•
Availableinmultiplecolors•
Idealforwesternblots,flowcytometry,imagingandmicroscopy,microplateassaysandmorePropertiesofQdot®NanocrystalsTheQdot®streptavidinconjugateisthesizeofalargemacromoleculeorprotein(~15–20nm)andrepresentsthebrightestclassofstreptavidindetectionreagents.Qdot®streptavidinconjugatesaremadefromananometer-scalecrystalofasemiconductormaterial(CdSe),whichiscoatedwithanadditionalsemiconductorshell(ZnS)toimprovetheopticalpropertiesofthematerial.TheQdot®705andQdot®800streptavidinconjugates,whichincludeCdSeTe,aremadeinasimilarfashion.Thiscore-shellmaterialisfurthercoatedwithapolymershellthatallowsthematerialstobeconjugatedto
BIOLOGicalmoleculesandtoretaintheiropticalproperties.
OtherFluorescentConjugatesofStreptavidinareAvailableWeofferseveralotherQdot®colorsortrytheQdot®StreptavidinSamplerKit,containingQdot®streptavidinconjugatesinsixcolors525,565,585,605,655,and705.Inadditiontonanocrystalconjugates,weofferawiderangeofstreptavidinsconjugatedtoAlexaFluor®dyes,OregonGreen®dye,enzymeconjugates,andtr
ADItionalfluorophoreslikeTexasRed®dye,fluorescein(FITC)andmore.
FindBiotinylatedConjugatesWeofferanextensivelineofbiotinylatedconjugatesforuseinbiotin-streptavidindetectionstrategies.
•Usetheprimaryantibodysearchtooltofindbiotinylatedprimaryantibodies
•Usethesecondaryantibodyselectortooltofindbiotinylatedsecondaryantibodiesandbiotinylatedanti-dyeandanti-haptenantibodies
BlockingEndogenousBiotinNaturallyoccurringbiotinscaninterferewithbiotin-streptavidindetectionschemes.Forexperimentsinvolvingfixedandperme
ABIlizedcells,tryourEndogenousBiotin-BlockingKittominimizethisinterference.
ForResearchUseOnly.Notintendedforanyanimalorhumantherapeuticordiagnosticuse.
RelatedLinks:LearnmoreaboutAvidin-BiotinDetection
TolearnmoreaboutQdot®Nanocrystals
ForResearchUseOnly.Notforuseindiagnosticprocedures.
購物車為空，快去購物吧！
4000-520-616
咨詢顧問
特別 提示
