• <menu id="uuusw"></menu>
    •
  • <table id="uuusw"></table>
    當前位置 ： NEB >>> NEB/BioLux? Cypridina Luciferase Assay Kit/E3309S/1,000 assays
    NEB/BioLux? Cypridina Luciferase Assay Kit/E3309S/1,000 assays
    • NEB/BioLux? Cypridina Luciferase Assay Kit/E3309S/1,000 assays

    NEB/BioLux? Cypridina Luciferase Assay Kit/E3309S/1,000 assays

    價格: ￥7536.00 市場價: 12560.00
    打印產品信息
    貨號: E3309S-1,000assays
    品牌: NEB
    規格
    數量
    庫存(0)
    特別 提示
    代購產品：無質量問題不接受退換貨，下單前請仔細核對信息。
    下單后請及時聯系客服核對商品價格，訂單生效后再付款。
    立即購買 加入購物車

    收藏商品
    資深產品顧問
    咨詢顧問

    全國免費服務熱線

    4000-520-616

    • 自營商城 一站式服務
    • 廠家直采 剔除溢價
    • 品質甄選 正品保證
    • 嚴控流程 只做188精品
    • 極速物流 如約送貨
    • 詳情
    • 使用說明
    • 常見問題

      • Description:

        TheBioLuxCypridinaLuciferaseAssayKitcontainsthereagentsnecessaryforassayingCypridinaLuciferaseactivity.CypridinaisanewsecretedluciferasereporterfromtheostracodCypridinanoctiluca.ThisluciferasedoesnotrequireATPandcatalyzestheoxidationofitsluciferinsubstrateinaphotochemicalreaction(1,2)(Figure1).ThesubstrateforCypridinaisdifferentthancoelenterazine,whichisthecommonsubstrateofothermarineluciferasesincludingRenillaandGaussia.

        PropertiesofCypridinaLuciferase:?

        CypridinaLuciferaseissecretedfromcellsbyvirtueofitsnaturalsignalpeptideanditsluminescencecanbemeasuredfromthesupernatantoftransfectedcells.Therefore,celllysisisnotnecessary(Figure2).?

        SecretedCLucisaverystableprotein.Becauseofthisproperty,theactivitymeasuredfromthesupernatantreflectstheamountofproteinaccumulateduptothetimeofsampling.Multiplesamplescanthereforebeobtainedfromthesametransfectedcells(Figure2).?

        TheCLucassayisverysensitive,allowingdetectionofverysmallamountsofCypridinaLuciferaseactivity(Figure3).?

        SecretedCLucisthermallystableat55°C(Figure4A),whichisthetypicalinactivationtemperatureofmostviruses.SecretedCLucremainsactiveinthepresenceofβ-mercaptoethanol,whichiscommonlypresentinthecompleteculturemediumofmouseEScells(Figure4B).?

        AlthoughthelightreactioncatalyzedbyCypridinahasaninitialemissionhalf-lifeofapproximately5minutes,lightproductioncontinuestodecayslowly,andisreADIlydetectable25minutesaftersubstrateaddition(Figure5).

        The0.5mlBioLuxCypridina?LuciferaseSubstrateSolventmustbecompletelythawedanddilutedwithabsoluteethanol(notincluded)beforeusingittomakethe100Xsubstratesolution(pleaserefertothesectionofReconstitutionofBioLuxCypridinaLuciferaseSubstrate).

        Figure1:ThephotochemicalreactioncatalyzedbyCypridinaLuciferase.Figure 1: The photochemical reaction catalyzed by Cypridina Luciferase.

        Figure2:ActivityofsecretedCLucatdifferenttimepoints.Figure 2: Activity of secreted CLuc at different time points.

        Supernatantsoftransfectedcellswerecollectedandmediumwasreplacedeachdayfor4days.Thesesupernatantswerestoredat-20°Cuntilthelastsampleswereobtained.RelativeLightUnits,RLU.
        Figure3:SensitivityofCypridinaLuciferaseassay.Figure 3: Sensitivity of Cypridina Luciferase assay.

        TheCLucactivitywasassayedfrom10-foldserialdilutionsofthesupernatantfromastableCLuc-expressingcell?line.
        Figure4:StABIlityofCypridinaLuciferase.Figure 4: Stability of Cypridina Luciferase.

        (A)SupernatantobtainedfromstableCLuc-expressingcellswasincubatedat55°Cor95°Cfor30minutesandallowedtocooltoroomtemperature(25°C).Five-foldserialdilutionsofsupernatantwereassayedforCLucactivity.Thecontrolisthesupernatantoftheparentalcellsfromwhichthestablecelllinewascreated.(B)SupernatantsfromstableCLuc-expressingcells,growninmediumwithorwithoutβ-mercaptoethanol,wereincubatedat37°Candassayedeachdayfor7days.
        Figure5:Kineticsoflightemission.Figure 5: Kinetics of light emission.

        TheCypridinaLuciferasekineticassaywasperformedwiththesupernatantfromHeLacellstransiently
        transfectedwithaCLuc-expressingvector.Theassaysolutionwaspreparedandincubatedatroom
        temperaturefor30minutesbeforeuse.
        Figure6:CypridinaLuciferaseAssaysFigure 6: Cypridina Luciferase Assays

        Figure7:ActivityofCypridinaLuciferaseinthesupernatantandlysatefromastableCLuc-expressingcellline.Figure 7: Activity of Cypridina Luciferase in the supernatant and lysate from a stable CLuc-expressing cell line

        TheCLucactivitywasmeasuredfrom20μlofsupernatant(from500μltotalvolume)andfrom20μlofcelllysate(100μltotallysatevolume)ofastableCLuc-expressingcellline.

        KitComponents

        Thefollowingreagentsaresuppliedwiththisproduct:

        Storeat(°C)Concentration
        BioLuxClucAssayBuffer1X
        BioLuxClucSubstrateSolvent
        lyophilizedBioLuxClucSubstrate

        Notes:

        StoretheBioLuxCypridinaLuciferaseAssayBuffer(1X)at-20°Cforupto2years.TheBioLuxCypridinaLuciferaseAssayBuffer(1X),thereconstitutedCLucsubstrate(100Xsolution)andtheCLucassaysolutionmustbeprotectedfromlight.TheCLucassaysolutionshouldbefreshlypreparedandincubatedatroomtemperaturefor30minutes(protectfromlight)beforeuse.Thelinearrangeoftheluminometermustbeestablished.ThiscanbeeasilydonebyassayingserialdilutionsofaCLuccontainingsample(Figure3),suchastheculturemediumofCLuc-expressingcells.Inaddition,theassaysolutionitselfaswellastheconditionedmedium(culturemediumfromuntransfectedcells)shouldbeincludedtoestablishthebackgroundintheassay.Ifexcessactivityfortheinstrumentrangeisfound,thesampleshouldbedilutedineitherPBSor10%serum-containingmedium.Theintegrationtimecanalsobereduced,e.g.2secondsinsteadof5seconds.Thepresenceofserum,i.e.culturemedium,typicallyincreasesthebackgroundsignalintheassay.Forexample,theCLucassaysolutionaloneshows101–102RLU;theCLucassaysolutionin10%FBS-containingmediumresultsin102–104RLU,dependingonthetypeofmediumused.Therecommendedintegrationtimefortheluminescencemeasurementis2–10seconds.Itisimportanttokeeptheintegrationtimeconstant,inordertoobtainconsistentresults.BioLuxCypridinaLuciferaseAssayBuffer(1X)andSubstrate(lyophilizedpowder)mustalwaysbeprotectedfromlight.BioLuxCypridinaLuciferaseSubstrateissuppliedinlyophilizedformtoensurelongshelflifeandmaximumstability.
      售后保障
      螞蟻淘生物188，秉承螞蟻淘一貫的嚴謹態度，由螞蟻淘公司專業人員負責品控、采購、物流、銷售、售后，保障正品優質。以“快速好省，為科研提供好產品、好價格”為理念，直接鏈接原廠家，從全國各地原制造商嚴格挑選188款科研精品，剔除品牌溢價，188生物新電商，把好的產品帶給科研！? 力求給你最優質的商品。
    • Q：生物188產品正品保障嗎？
      A：生物188質量把控人員具有十年的從業經驗，在業界享有良好的口碑；自營商城，直接從廠家采購， 自己的團隊負責國際物流和清關，中間沒有第三方，所有流程嚴格把控，100%保證正品，假一罰十。

      Q：下單后可以修改訂單嗎？
      A：下單后的商品付款之前可以修改；訂單付款成功，需要聯系我們客服進行修改；客服電話：4000-520-616

      Q：可以開發票嗎？
      A：本網站所售商品都是正規清關，均開具16%正規發票，發票金額含配送費金額，另有說明的除外。

      Q：商品幾天可以發貨？
      A：生物188商品，全部現貨銷售，付款后即可發貨,一般一周內送達！

      Q：如何聯系商家？
      A：有任何問題夠可以電話咨詢我們，全國24小時免費服務熱線：4000-520-616 或聯系我們的在線客服QQ：1570468124

      Q：收到的商品少了/發錯了怎么辦？
      A：同個訂單購買多個商品可能會分為一個以上包裹發出，可能不會同時送達，建議查看訂單詳情是否是 部分發貨狀態；如未收到，可聯系在線客服或者致電4000-520-616。

      Q：退換貨/維修需要多長時間？
      A：一般情況下，退貨處理周期為客戶收到產品一個月內（以快遞公司顯示簽收時間為準），包裝規格、 數量、品種不符，外觀毀損、短缺或缺陷，請在收到貨24小時內申請退換貨；特殊商品以合同條款為準。

    客戶服務

    在線客服
    用戶反饋

    何為188

    極簡而嚴謹，我們僅銷售188款生物醫學科研用品，款款都是爆款；因為少所以聚焦，聚焦甄選每一款產品，聚焦服務每一位客戶!

    關注我們 :

    點擊QQ聯系我們
    生物188微信

    關注188微信公眾號

    獲取最新優惠活動通知

    • 品質甄選，正品保證

    • 自營電商，廠家直采

    • 極簡主義，188精品

    常見問題 | 購買相關 | 配送與驗收 | 支付問題 | 售后與服務 | 服務協議 | 隱私政策 | 何為188 | 中國智造 | 聯系我們
    友情鏈接： ImmunoReagents產品 | PelFreez-Bio價格 | ultraclean產品代理 | maxvisionbio官網 | ezbiosystems代理 | 螞蟻淘 | 細胞培養瓶

    公司版權所有 ? 2005-2021 蘇州螞蟻淘生物科技有限公司 蘇ICP備17049038號-10

  • <menu id="uuusw"></menu>
    •
  • <table id="uuusw"></table>
    上床视频