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    當前位置 : Millipore >>> Millipore/ABE257 | Anti-RAD51 Antibody/ABE257/100 µL
    Millipore/ABE257 | Anti-RAD51 Antibody/ABE257/100 µL
    • Millipore/ABE257 | Anti-RAD51 Antibody/ABE257/100 µL

    Millipore/ABE257 | Anti-RAD51 Antibody/ABE257/100 µL

    價格: ¥3324.00 市場價: 5540.00

    貨號: ABE257
    品牌: Millipore
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      • Description
        CatalogueNumberABE257
        ReplacesMABE515
        DescriptionAnti-RAD51Antibody
        AlternateNames
        • DNArepairproteinRAD51homolog1
          Shortname=HsRAD51
          Shortname=hRAD51
          Alternativename(s):
          RAD51homologA
        BackgroundInformationRAD51isessentialforhomologousrecombinationofDNAduringrepairofdouble-strandbreaks.TheN-terminusofRAD51functionstobindDNAandpromotehomologouspairingandstrandexchangewithinaregularnucleoproteinfilament.Inyeast,theRad51familyofrelatedproteinsalsoincludesRad55,Rad57,andDmc1.Inmammaliancells,sevengenesinthisfamilyhavebeenidentified(HsRAD51,XRCC2,XRCC3,RAD51B/hREC2andHsDMC1,RAD51C,andRAD51D).RAD51ispredominantlyexpressedinthetestisandthymusbutcanalsobefoundinthesmallintestine,placenta,colon,pancreasandovaries.
        ProductInformation
        FormatAffinityPurified
        Control
        • HeLacelllysate
        PresentationPurifiedrabbitpolyclonalinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazide.
        StorageandShippingInformation
        StorageConditionsStablefor1yearat2-8°Cfromdateofreceipt.
        Applications
        ApplicationAnti-RAD51AntibodyisaRabbitPolyclonalAntibodyfordetectionofRAD51alsoknownasDNArepairproteinRAD51homolog1,HsRAD51&hasbeenvalidatedinWB,ICC&IP.
        KeyApplications
        • WesternBlotting
        • Immunocytochemistry
        • Immunoprecipitation
        ApplicationNotesImmunocytochemistryAnalysis:1:500dilutionfromarepresentativelotdetectedRAD51inA431andHeLacells.

        ImmunoprecipitationAnalysis:40μLofAnti-RAD51immunoprecipitatedRAD51fromHeLaRIPAlysate.
        BIOLOGicalInformation
        ImmunogenHistidine-taggedrecombinantproteincorrespondingtohumanRAD51.
        ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
        HostRabbit
        SpeciesReactivity
        • Human
        • Mouse
        • Rat
        SpeciesReactivityNoteDemonstratedtoreactwithHuman,Mouse,andRat.Otherhomologies:Bovine,Equine,andCanine(99%sequencehomology).
        AntibodyTypePolyclonalAntibody
        EntrezGeneNumber
        EntrezGeneSummaryTheproteinencodedbythisgeneisamemberoftheRAD51proteinfamily.RAD51familymembersarehighlysimilartobacterialRecAandSaccharomycescerevisiaeRad51,andareknowntobeinvolvedinthehomologousrecombinationandrepairofDNA.ThisproteincaninteractwiththessDNA-bindingproteinRPAandRAD52,anditisthoughttoplayrolesinhomologouspairingandstrandtransferofDNA.ThisproteinisalsofoundtointeractwithBRCA1andBRCA2,whichmaybeimportantforthecellularresponsetoDNAdamage.BRCA2isshowntoregulateboththeintracellularlocalizationandDNA-bindingABIlityofthisprotein.LossofthesecontrolsfollowingBRCA2inactivationmaybeakeyeventleADIngtogenomicinstabilityandtumOrigenesis.Multipletranscriptvariantsencodingdifferentisoformshavebeenfoundforthisgene.[providedbyRefSeq].
        GeneSymbol
        • RAD51
        • RAD51A
        • RECA
        PurificationMethodAffinityPurfied
        UniProtNumber
        UniProtSummaryFUNCTION:MayparticipateinacommonDNAdamageresponsepathwayassociatedwiththeactivationofhomologousrecombinationanddouble-strandbreakrepair.BindstosingleanddoublestrandedDNAandexhibitsDNA-dependentATPaseactivity.UnderwindsduplexDNAandformshelicalnucleoproteinfilaments.PlaysaroleinregulatingmitochondrialDNAcopynumberunderconditionsofoxidativestressinthepresenceofRAD51CandXRCC3.

        SUBUNITSTRUCTURE:InteractswithBRCA1,BRCA2andeitherdirectlyorindirectlywithp53.InteractswithXRCC3,RAD54LandRAD54B.PartofacomplexwithRAD51CandRAD51B.InteractswithRAD51AP1andRAD51AP2.InteractswithCHEK1/CHK1,andthismayrequirepriorphosphorylationofCHEK1.InteractswiththeMND1-PSMC3IPheterodimer(Bysimilarity).InteractswithOBFC2B.InteractswithRPA1.InteractswithPALB2.InteractswithSWI5/C9orf119,andatlowerlevelwithSFR1/MEIR5.

        SUBCELLULARLOCATION:Nucleus.Cytoplasm.Cytoplasm?perinuclearregion.Mitochondrionmatrix.Note:ColocalizeswithRAD51AP1tomultiplenuclearfociuponinductionofDNAdamage.DNAdamageinducesanincreaseinnuclearlevels.

        TISSUESPECIFICITY:Highlyexpressedintestisandthymus,followedbysmallintestine,placenta,colon,pancreasandovary.Weaklyexpressedinbreast.

        INDUCTION:Stress-inducedincreaseinthemitochondriallevelsisseen.

        PTM:Phosphorylated.PhosphorylationofThr-309byCHEK1/CHK1mayenhanceassociationwithchromatinatsitesofDNAdamageandpromoteDNArepairbyhomologousrecombination.

        INVOLVEMENTINDISEASE:DefectsinRAD51areacauseofsusceptibilitytobreastcancer(BC)[MIM:114480].Acommonmalignancyoriginatingfrombreastepithelialtissue.Breastneoplasmscanbedistinguishedbytheirhistologicpattern.Invasiveductalcarcinomaisbyfarthemostcommontype.Breastcancerisetiologicallyandgeneticallyheterogeneous.Importantgeneticfactorshavebeenindicatedbyfamilialoccurrenceandbilateralinvolvement.Mutationsatmorethanonelocuscanbeinvolvedindifferentfamiliesoreveninthesamecase.Ref.28

        SEQUENCESIMILARITIES:BelongstotheRecAfamily.RAD51subfamily.

        Contains1HhHdomain.
        MolecularWeight~37kDaobserved.Twoisoforms(isoform1at37kDaandisoform2at26kDa)producedasaresultofalternativesplicingareknowntoexist:.
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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