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    當前位置 ： Millipore >>> Millipore/17-651 | ChIPAb+ Monomethyl-Histone H4 (Lys20) - ChIP Validated Antibody and Primer Set/17-651/25 assays25 ass
    Millipore/17-651 | ChIPAb+ Monomethyl-Histone H4 (Lys20) - ChIP Validated Antibody and Primer Set/17-651/25 assays25 ass
    • Millipore/17-651 | ChIPAb+ Monomethyl-Histone H4 (Lys20) - ChIP Validated Antibody and Primer Set/17-651/25 assays25 ass

    Millipore/17-651 | ChIPAb+ Monomethyl-Histone H4 (Lys20) - ChIP Validated Antibody and Primer Set/17-651/25 assays25 ass

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    貨號: 17-651
    品牌: Millipore
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      • Description
        CatalogueNumber17-651
        BrandFamilyUpstate
        TradeName
        • ChIPAb+
        • Upstate
        DescriptionChIPAb+Monomethyl-HistoneH4(Lys20)-ChIPValidatedAntibodyandPrimerSet
        OverviewAllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction.
        TheChIPAb+Monomethyl-HistoneH4(Lys20)setincludestheAnti-monomethyl-HistoneH4(Lys20)antibody,anegativecontrolantibody(purifiedRabbitIgG),andqPCRprimers,whichamplifya212basepairPCRproductwithinthehumanGAPDHcodingregion.ThemonomethylhistoneH4(Lys20)andnegativecontrolantibodiesaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofmonomethyl-histoneH4(Lys20)associatedchromatin.
        AlternateNames
        • H4K20me1
        • HistoneH4(monomethylK20)
        • H4histonefamily,memberA
        • histone1
        • H4a
        • histonecluster1
        • H4a
        ProductInformation
        FormatAffinityPurified
        Control
        • IncludesnegativecontrolrabbitIgGantibodyandcontrolprimersspecificforhumanGAPDHcodingregion.
        PresentationAnti-monomethyl-HistoneH4(Lys20)(rabbitpolyclonalIgG).Onevialcontaining100μgofaffinitypurifiedantibodyin100μL0.1MTris-Glycine(pH7.4),15mMNaCl,and0.05%NaN3.Storeat-20°C.

        NormalRabbitIgG.Onevialcontaining125μgofpurifiedrabbitIgGin125μLstoragebuffer.Storeat-20°C.

        ChIPPrimersGAPDHCodingRegion.Onevialcontaining75μLof5μMofeachprimerspecificforhumanGAPDHcodingregion.Storeat-20°C.
        FOR:GGCTCCCACCTTTCTCATCC
        REV:GGCCATCCACAGTCTTCTGG
        StorageandShippingInformation
        StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
        Applications
        ApplicationThisChIPAb+Monomethyl-HistoneH4(Lys20)-ChIPValidatedAntibody&PrimerSetconvenientlyincludestheantibody&thespecificcontrolPCRprimers.
        KeyApplications
        • WesternBlotting
        • ChromatinImmunoprecipitation(ChIP)
        ApplicationNotesChromatinImmunoprecipitation:
        SonicatedchromatinpreparedfromHeLacells(2X106cellequivalentsperIP)wassubjectedtochromatinimmunoprecipitationusing4μgofeitheranegativecontrolantibodyorAnti-Monomethyl-HistoneH4
        (Lys20)antibodyandtheMagnaChIPAKit(Cat.#17-610).Successfulimmunoprecipitationofmonomethyl-histoneH4(Lys20)-associatedDNAfragmentswasverifiedbyqPCRusingGAPDHcodingregionChIPPrimersversusControlPrimerscorrespondingtotheGAPDHpromoter(Pleaseseefigures).DataispresentedaspercentinputofeachIPsamplerelativetoinputchromatin,withimmunoprecipitatedDNAfromnegativecontrolantibodyshownas(-)andmonomethyl-histoneH4(Lys20)shownas(+).
        PleaserefertotheEZ-MagnaChIP?A(Cat.#17-408)orEZ-ChIP?(Cat.#17-371)protocolforexperimentaldetails.

        WesternBlotAnalysis:
        0.1μg/mLofproteinswerevisualizedusingagoatanti-rabbitsecondaryantibodyconjugatedtoHRPandachemiluminescencedetectionsystem(Pleaseseefigures)

        PeptideBlockingAssay:
        40μgofhistoneH4peptidecontainingmonomethylLys20abolisheddetectionofhistoneH4byanti-monomethyl-HistoneH4(Lys20)inimmunoblotanalysisofacidextractsofHeLacells(Pleaseseefigures).
        BiologicalInformation
        ImmunogenTheimmunogenwasasyntheticpeptidecorrespondingtoaminoacids15-24ofhistoneH4,monomethylatedonLys20.
        Epitopea.a.15-24
        HostRabbit
        SpecificityRecognizeshistoneH4,Mr~11kDa.
        SpeciesReactivity
        • Human
        • GreenPlants
        • Vertebrates
        SpeciesReactivityNoteHuman.Nottestedinotherspecies.Theimmunogensequenceisidenticalinawiderangeofanimalandplantspecies,sobroadcross-reactivityisexpected.
        AntibodyTypePolyclonalAntibody
        EntrezGeneNumber
        EntrezGeneSummaryHistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Twomoleculesofeachofthefourcorehistones(H2A,H2B,H3,andH4)formanoctamer,aroundwhichapproximately146bpofDNAiswrappedinrepeatingunits,callednucleosomes.Thelinkerhistone,H1,interactswithlinkerDNAbetweennucleosomesandfunctionsinthecompactionofchromatinintohigherorderstructures.ThisgeneisintronlessandencodesamemberofthehistoneH4family.TranscriptsfromthisgenelackpolyAtailsbutinsteadcontainapalindromicterminationelement.Thisgeneisfoundinthelargehistonegeneclusteronchromosome6.
        GeneSymbol
        • H4/A
        • H4/B
        • H4/C
        • H4/D
        • H4/E
        • H4/G
        • H4/H
        • H4/I
        • H4/J
        • H4/J
        • H4/M
        • H4/N
        • H4/O
        • H4/a
        • H4F2
        • H4FA
        • H4FB
        • H4FC
        • H4FD
        • H4FE
        • H4FG
        • H4FH
        • H4FI
        • H4FJ
        • H4FK
        • H4FM
        • H4FN
        • H4FO
        • HIST2H4
        Modifications
        • Methylation
        UniProtNumber
        UniProtSummaryFUNCTION:Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.

        SUBUNITSTRUCTURE:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.

        SUBCELLULARLOCATION:Nucleus.

        PTM:AcetylationatLys-6,Lys-9,Lys-13andLys-17occursincodingregionsofthegenomebutnotinheterochromatin.

        CitrullinationatArg-4byPADI4impairsmethylation.

        MonomethylationatArg-4byPRMT1favorsacetylationatLys-9andLys-13.DemethylationisperformedbyJMJD6.

        Monomethylated,dimethylatedortrimethylatedatLys-21.MonomethylationisperformedbySET8.TrimethylationisperformedbySUV420H1andSUV420H2andinducesgenesilencing.

        UbiquitinatedbytheCUL4-DDB-RBX1complexinresponsetoultravioletirradiation.ThismayweakentheinteractionbetweenhistonesandDNAandfacilitateDNAaccessibilitytorepairproteins.

        Sumoylated,whichisassociatedwithtranscriptionalrepression.

        SEQUENCESIMILARITY:BelongstothehistoneH4family.

        SEQUENCECAUTION:ThesequenceAAI28106.1differsfromthatshown.Reason:Frameshiftatposition3.

        MolecularWeight~11kDa
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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