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    當前位置 ： Millipore >>> Millipore/17-600 | ChIPAb+ CREB - ChIP Validated Antibody and Primer Set, rabbit monoclonal/17-600/25 assays25 assays pe
    Millipore/17-600 | ChIPAb+ CREB - ChIP Validated Antibody and Primer Set, rabbit monoclonal/17-600/25 assays25 assays pe
    • Millipore/17-600 | ChIPAb+ CREB - ChIP Validated Antibody and Primer Set, rabbit monoclonal/17-600/25 assays25 assays pe

    Millipore/17-600 | ChIPAb+ CREB - ChIP Validated Antibody and Primer Set, rabbit monoclonal/17-600/25 assays25 assays pe

    價格: ￥4800.00 市場價: 8000.00
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    貨號: 17-600
    品牌: Millipore
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      • Description
        CatalogueNumber17-600
        BrandFamilyUpstate
        TradeName
        • ChIPAb+
        • Upstate
        DescriptionChIPAb+CREB-ChIPValidatedAntibodyandPrimerSet,rabbitmonoclonal
        OverviewEverylotoftheChIPAb+lineofantibodiesisindividuallyvalidatedforchromatinprecipitation,inordertoguaranteesuccessfulChIPassayseverytime.Eachantibodyincludesacontrolprimersetforperformanceconfirmation.CREBantibodyandthenegativecontrolantibody(rabbitnormalIgG)canbeusedtodemonstratethattheCREBantibodyisfunctionallyvalidatedintheprecipitationofCREBassociatedchromatin.
        TheqPCRprimersincludedflanktheCREBbindingsiteinhumanNR4A2promoter.
        AlternateNames
        • activetranscriptionfactorCREB
        • cAMPresponsiveelementbindingprotein1
        • cAMP-responseelement-bindingprotein-1
        • cAMP-responsiveelement-bindingprotein1
        • transactivatorprotein
        BackgroundInformationAllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction.
        TheChIPAb+CREB,antibody/primersetincludestheCREB,antibody,anegativecontrolantibody(purifiedrabbitIgG),andqPCRprimerswhichamplifya64bpregionflankingtheCREofthehumancFosgene.TheCREBandnegativecontrolculturedsupernatantaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofCREBassociatedchromatin.
        ProductInformation
        Components
        • Anti-CREB(RabbitmonoclonalIgG):PartNo.CS203204.Onevialcontaining250μLofculturedsupernatantcontaining0.05%sodiumazide.Storeat-20°C.
          NegativeControlSupernatant:PartNo.CS203202.Onevialcontaining250μLofculturedsupernatantcontaining0.05%sodiumazide.Storeat-20°C.
          ChIPPrimerscFosCRE:PartNo.CS203203.Onevialcontaining75μLof5μMofeachprimerspecificforthecFosCRE.Storeat-20°C.
          FOR:GGCCCACGAGACCTCTGAGACA
          REV:GCCTTGGCGCGTGTCCTAATCT
        FormatCultureSupernatant
        Control
        • IncludesnegativecontrolculturedsupernatantandprimersspecificforofhumancFosCRE.
        Presentation25assaysperset.Recommendeduse:~10μLantibodyperchromatinimmunoprecipitation(dependentuponbiologicalcontext).
        StorageandShippingInformation
        StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
        Applications
        ApplicationThisChIPAb+CREB-ChIPValidatedAntibody&PrimerSetconvenientlyincludestheantibody&thespecificcontrolPCRprimers.
        KeyApplications
        • WesternBlotting
        • ChromatinImmunoprecipitation(ChIP)
        ApplicationNotesChromatinImmunoprecipitation:
        Representativelotdata.
        Sonicatedchromatinpreparedfrom293Tcells(5X106cellequivalentsperIP)wassubjectedtochromatinimmunoprecipitationusing10μLofeitheranegativesupernatant,orCREBantibodyandtheMagnaChIP?AKit(Cat.#17-610).
        SuccessfulimmunoprecipitationofCREB-associatedDNAfragmentswasverifiedbyqPCRusingcFosdownstream4Kb(negative)andcFosCRE(positive)(Figure2).DataispresentedaspercentinputofeachIPsamplerelativetoinputchromatinforeachampliconandChIPsampleasindicated.
        PleaserefertotheEZ-MagnaAChIP?(Cat.#17-408)orEZ-ChIP?(Cat.#17-371)protocolforexperimentaldetails.

        WesternBlotAnalysis:
        Representativelotdata.
        HeLanuclearextractwasresolvedbyelectrophoresis,transferredtonitrocelluloseandprobedwithanti-CREB,cloneNL904(1:8,000dilution)
        Proteinswerevisualizedusingagoatanti-rabbitsecondaryantibodyconjugatedtoHRPandachemiluminescencedetectionsystem.
        ArrowindicatesCREB(~43kDa).
        BiologicalInformation
        ImmunogenCREBpurifiedantibodyismadeagainstaKLH-conjugatedsyntheticpeptidecorre-spondingtoaminoacids5-24ofhumanCREB.
        Epitopea.a.5-24
        CloneNL904
        HostRabbit
        SpecificityRecognizesCREB,MW~43kDa.
        IsotypeIgG
        SpeciesReactivity
        • Human
        • Mouse
        • Rat
        SpeciesReactivityNoteHuman,mouse,rat.Nottestedinotherspecies.Theimmunogensequenceisidenticalinawiderangeofanimalandplantspecies,sobroadcross-reactivityisexpected.
        AntibodyTypeMonoclonalAntibody
        EntrezGeneNumber
        EntrezGeneSummaryThisgeneencodesatranscriptionfactorthatisamemberoftheleucinezipperfamilyofDNAbindingproteins.ThisproteinbindsasahomodimertothecAMP-responsiveelement,anoctamericpalindrome.Theproteinisphosphorylatedbyseveralproteinkinases,andinducestranscriptionofgenesinresponsetohormonalstimulationofthecAMPpathway.Alternatesplicingofthisgeneresultsintwotranscriptvariantsencodingdifferentisoforms.
        GeneSymbol
        • CREB1
        • MGC9284
        • CREB
        UniProtNumber
        UniProtSummaryFUNCTION:ThisproteinbindsthecAMPresponseelement(CRE),asequencepresentinmanyviralandcellularpromoters.CREBstimulatestranscriptiononbindingtotheCRE.TransciptionactivationisenhancedbytheTORCcoactivatorswhichactindependentlyofSer-133phosphorylation.ImplicatedinsynchronizationofcircADIanrhythmicity.
        SUBUNITSTRUCTURE:InteractswithPPRC1.BindsDNAasadimer.ThisdimerisstABIlizedbymagnesiumions.Interacts,throughthebZIPdomain,withthecoactivatorsTORC1/CRTC1,TORC2/CRTC2andTORC3/CRTC3.WhenphosphorylatedonSer-133,bindsCREBBPBysimilarity.
        SUBCELLULARLOCATION:Nucleus.
        PTM:Stimulatedbyphosphorylation.PhosphorylationofbothSer-133andSer-142intheSCNregulatestheactivityofCREBandparticipatesincircadianrhythmgeneration.PhosphorylationofSer-133allowsCREBBPbindingBysimilarity.PhosphorylateduponDNAdamage,probablybyATMorATR.
        INVOLVEMENTINDISEASE:AchromosomalaberrationinvolvingCREB1isassociatedwithangiomatoidfibroushistiocytoma(AFH)[MIM:612160].Translocationt(2;22)(q33;q12)withCREB1generatesaEWSR1/CREB1fusiongenethatismostcommongeneticabnormalityinthistumortype.
        SEQUENCESIMILARITIES:BelongstothebZIPfamily.Contains1bZIPdomain.Contains1KID(kinase-inducIBLe)domain.
        MolecularWeight~43kDa
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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