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    當前位置 ： Millipore >>> Millipore/17-10131 | ChIPAb+? Phospho-CREB (Ser133) - ChIP Validated Antibody and Primer Set/17-10131/25 assays25 assays
    Millipore/17-10131 | ChIPAb+? Phospho-CREB (Ser133) - ChIP Validated Antibody and Primer Set/17-10131/25 assays25 assays
    • Millipore/17-10131 | ChIPAb+? Phospho-CREB (Ser133) - ChIP Validated Antibody and Primer Set/17-10131/25 assays25 assays

    Millipore/17-10131 | ChIPAb+? Phospho-CREB (Ser133) - ChIP Validated Antibody and Primer Set/17-10131/25 assays25 assays

    價格: ￥4800.00 市場價: 8000.00
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    貨號: 17-10131
    品牌: Millipore
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      • Description
        CatalogueNumber17-10131
        TradeName
        • ChIPAb+
        • Upstate
        DescriptionChIPAb+?Phospho-CREB(Ser133)-ChIPValidatedAntibodyandPrimerSet
        OverviewAllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction.
        TheChIPAb+Phospho-CREB(Ser133)setincludesthePhospho-CREB(Ser133)antibody,anegativecontrolnormalrabbitIgG,andqPCRprimerswhichamplifya64bpregionofhumancFosCRE.ThePhospho-CREB(Ser133)andnegativecontrolsaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofPhospho-CREB(Ser133)-associatedchromatin.
        AlternateNames
        • activetranscriptionfactorCREB
        • cAMPresponsiveelementbindingprotein1
        • cAMP-responseelement-bindingprotein-1
        • transactivatorprotein
        BackgroundInformationCREBisaβ-ZIPtranscriptionfactorthatactivatestargetgenesthroughcAMPresponseelements.CREBisabletomediatesignalsfromnumerousphysiologicalstimuli,resultinginregulationofabroadarrayofcellularresponses.WhileCREBisexpressedinnumeroustissues,itplaysalargeregulatoryroleinthenervoussystem.CREBisbelievedtoplayakeyroleinpromotingneuronalsurvival,precursorproliferation,neuriteoutgrowthandneuronaldifferentiationincertainneuronalpopulations.CREBpromotesoutgrowthanddifferentiationasamediatorofneurotrophinpathways.CREBisabletoselectivelyactivatenumerousdownstreamgenesthroughinteractionswithdifferentdimerizationpartners.CREBisactivatedbyphosphorylationatSer133byvarioussignalingpathwaysincludingErk,Ca2+andstresssignaling.
        ProductInformation
        FormatPurified
        Control
        • IncludesnegativecontrolnormalrabbitIgGandprimersspecificforhumancFOSCRE.
        PresentationAnti-phospho-CREB(Ser133)(rabbitpolyclonal),PartNo.CS204400.Onevialcontaining100μgofaffinitypurifiedrabbitserumin0.1MTris-Glycine(pH7.4)0.15MNaCl,and0.05%sodiumazide,beforetheadditionof30%glycerol.Storeat-20°C.
        NormalRabbitIgG,PartNo.PP64B.Onevialcontaining125μgRabbitIgGin125μLstoragebuffercontaining0.05%sodiumazide.
        Storeat-20°C.
        ChIPPrimerscFosCRE,PartNo.CS203203.Onevialcontaining75μLof5μMofeachprimerspecificforthecFosCRE.Storeat-20°C.
        FOR:GGCCCACGAGACCTCTGAGACA
        REV:GCCTTGGCGCGTGTCCTAATCT
        StorageandShippingInformation
        StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.
        Applications
        ApplicationThisChIPAb+Phospho-CREB(Ser133)-ChIPValidatedAntibody&PrimerSet(AntibodyChIP)convenientlyincludestheantibody&thespecificcontrolPCRprimers.
        KeyApplications
        • ElectrophoreticMobilityShiftAssay
        • Immunohistochemistry
        • Immunoprecipitation
        • WesternBlotting
        • ChromatinImmunoprecipitation(ChIP)
        ApplicationNotesChromatinImmunoprecipitation:
        Representativelotdata.
        Sonicatedchromatinpreparedfrom293Tcells(5X10E6cellequivalentsperIP)weresubjectedtochromatinimmunoprecipitationusingeither4μgofNormalRabbitIgG,(PartNo.P64B),or4μgofAnti-Phospho-CREB(Ser133)antibody(PartNo.CS204400)andtheMagnaChIP?AKit(Cat.#17-610).
        SuccessfulimmunoprecipitationofPhospho-CREB(Ser133)antibodyassociatedDNAfragmentswasverifiedbyqPCRusingChIPPrimerscFosCRE(PartNo.CS203203)asapositivelocus,andcFosdownstream4Kbasanegativelocus(Figure2).DataispresentedaspercentinputofeachIPsamplerelativetoinputchromatinforeachampliconandChIPsampleasindicated.
        PleaserefertotheEZ-MagnaChIP?A(Cat.#17-408)orEZ-ChIP?(Cat.#17-371)protocolforexperimentaldetails.
        WesternBlotAnalysis:
        Representativelotdata.
        Untreated(lane1)andForskolin-treated(Lane2)NIH/3T3lysateswereresolvedbySDS-PAGE,transferredtoPVDF,andprobedwithanti-phospho-CREB(Ser133)(1:1,000dilution).
        Proteinswerevisualizedusingadonkeyanti-rabbitsecondaryantibodyconjugatedtoHRPandachemiluminescencedetectionsystem.
        ArrowindicatesphosphorylatedCREB(Figure3).
        Immunoprecipitation:
        10μLfromarepresentativelotofthisantibodywasshowntoimmunoprecipitatephosphorylatedCREBfrom500μgofcellstreatedwith50mMforskolin.
        Immunohistochemistry:A1:1000dilutionofarepresentativelotdetectedphosphorylatedCREBinformalin-fixedparaffin-embeddednormalandischemicratbrainsections.
        ElectrophoreticMobilityShift:
        Arepresentativelotofthisantibodyhasbeenshowntogelshiftbyanindependentlaboratory.
        BiologicalInformation
        ImmunogenKLH-conjugatedsyntheticpeptidecorrespondingtotheaminoacidsincludingandencompassingphosphorylatedSer133ofCREB.
        Epitopephospho-Ser133
        ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
        HostRabbit
        SpecificityRecognizesp43phosphorylatedCREB.Mayalsorecognizep30andp38kDaproteins.Thep30&p38proteinsmaybephosphorylatedCREMandATF-1respectively,sincethetwoproteinssharesignificanthomologytothephosphorylatedCREBimmunizingpeptide.
        IsotypeIgG
        SpeciesReactivity
        • Human
        • Rat
        • Mouse
        • Hamster
        AntibodyTypePolyclonalAntibody
        EntrezGeneNumber
        EntrezGeneSummaryThisgeneencodesatranscriptionfactorthatisamemberoftheleucinezipperfamilyofDNAbindingproteins.ThisproteinbindsasahomodimertothecAMP-responsiveelement,anoctamericpalindrome.Theproteinisphosphorylatedbyseveralproteinkinases,andinducestranscriptionofgenesinresponsetohormonalstimulationofthecAMPpathway.Alternatesplicingofthisgeneresultsintwotranscriptvariantsencodingdifferentisoforms.
        GeneSymbol
        • CREB1
        • CREB
        • MGC9284
        Modifications
        • Phosphorylation
        PurificationMethodPurifiedbyproteinA-SepharosefollowedbyimmunoadsorbantchromatographyusinganunphosphorylatedCREBaffinitygelcolumn.
        UniProtNumber
        UniProtSummaryFUNCTION:ThisproteinbindsthecAMPresponseelement(CRE),asequencepresentinmanyviralandcellularpromoters.CREBstimulatestranscriptiononbindingtotheCRE.TransciptionactivationisenhancedbytheTORCcoactivatorswhichactindependentlyofSer-133phosphorylation.ImplicatedinsynchronizationofcircADIanrhythmicity.
        SUBUNIT:InteractswithPPRC1.BindsDNAasadimer.Thisdimerisstabilizedbymagnesiumions.Interacts,throughthebZIPdomain,withthecoactivatorsTORC1/CRTC1,TORC2/CRTC2andTORC3/CRTC3.WhenphosphorylatedonSer-133,bindsCREBBPBysimilarity.
        SUBCELLULAR:Nucleus.
        PMT:Stimulatedbyphosphorylation.PhosphorylationofbothSer-133andSer-142intheSCNregulatestheactivityofCREBandparticipatesincircadianrhythmgeneration.PhosphorylationofSer-133allowsCREBBPbindingBysimilarity.PhosphorylateduponDNAdamage,probablybyATMorATR.
        SIMILARITY:BelongstothebZIPfamily.
        Contains1bZIPdomain.
        Contains1KID(kinase-inducIBLe)domain
        MolecularWeightDetectsCREBonlywhenphosphorylatedonSer133(~43kDa).MayrecognizeATF-1(~38kDa)andCREM(~30kDa)asthetwoproteinssharesignificanthomologytothephosphorylatedimmunizingpeptide.
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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